Beschreibung
Produktdetails
Einband
Taschenbuch
Erscheinungsdatum
04.07.1997
Verlag
John Wiley & Sons IncSeitenzahl
160
Maße (L/B/H)
23/16,1/1,5 cm
Gewicht
272 g
Auflage
1. Auflage
Sprache
Englisch
ISBN
978-0-471-15946-9
In situ gene amplification techniques offer tremendous potential as aids to clinical diagnosis through their ability to detect a single copy of a specific microbial, neoplastic, messenger, or mutated nucleic acid sequence in a cell smear, cell suspension, tissue section, or chromosome. In situ hybridization, applied in combination with the polymerase chain reaction (PCR), can be expected to fuel accelerated developments in the understanding of embryogenesis, organogenesis, and cell differentiation, as well as the pathogenesis of numerous disease processes. But the procedures are cumbersome and fraught with potential variables, and experimental results are difficult to reproduce.In Situ PCR Techniques addresses this problem directly, with comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant, insect, and prokaryotic cells. They facilitate the repetition of published experiments by providing the kinds of hints, tips, and laboratory secrets that are often left out of scientific papers. They also demonstrate clearly and thoroughly the efficacy of in situ PCR as an alternative to standard and fluorescence in situ hybridization for detecting a single copy of a mutant gene, a virus, or a very low-abundance message in individual cells, while preserving the morphology of the cell and the tissue in which it resides.
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