Flower Development (eBook, PDF)

Methods and Protocols
Redaktion: Riechmann, José Luis; Wellmer, Frank

• Format: PDF

Produktbeschreibung

In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls

Authoritative and practical, Flower Development: Methods and Protocols is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field.

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Produktdetails

• Verlag: Springer New York
• Seitenzahl: 475
• Erscheinungstermin: 6. Januar 2014
• Englisch
• ISBN-13: 9781461494089
• Artikelnr.: 64700867

Inhaltsangabe

Flower Development in Arabidopsis - There is More to it than Learning your ABCs.- Flower Development in the Asterid Lineage.- Grass Flower Development.- Flower Diversity and Angiosperm Diversification.- Flower Development - Open Questions and Future Directions.- Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors.- Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development.- Genetic and Phenotypic Analyses of Petal Development in Arabidopsis.- Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice.- Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana.- Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis.- Microscopic Analysis of Arabidopsis Ovules.- Scanning Electron Microscopy Analysis of Floral Development.- Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue.- The GUS Reporter System in Flower Development Studies.- A Floral Induction System for the Study of Early Arabidopsis Flower Development.- Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types.- Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers.- Laser-assisted Microdissection Applied to Floral Tissues.- Identification of Arabidopsis Knock-out Lines for Genes of Interest.- Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues.- Misexpression Approaches for the Manipulation of Flower Development.- Next Generation Sequencing Applied to Flower Development: RNA-Seq.- Next-Generation Sequencing Applied to Flower Development: ChIP-Seq.- Live-Imaging of the Arabidopsis Inflorescence Meristem.- Gene Regulatory Network Models for Floral Organ Determination.