Key Experiments in Practical Developmental Biology (eBook, PDF)

Redaktion: Mari-Beffa, Manuel

• Format: PDF

Produktbeschreibung

Originally published in 2005, this unique resource presents 27 easy-to-follow laboratory exercises for use in student practical classes in developmental biology. These experiments provide key insights into developmental questions, and many of them are described by the leaders in the field who carried out the original research. This book intends to bridge the gap between experimental work and the laboratory classes taken at the undergraduate and post-graduate levels. All chapters follow the same format, taking the students from materials and methods, through results and discussion, so that they learn the underlying rationale and analysis employed in the research. The book will be an invaluable resource for graduate students and instructors teaching practical developmental biology courses. Chapters include teaching concepts, discussion of the degree of difficulty of each experiment, potential sources of failure, as well as the time required for each experiment to be carried out in a class with students.

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Produktdetails

• Verlag: Cambridge University Press
• Erscheinungstermin: 17. Februar 2011
• Englisch
• ISBN-13: 9780511079153
• Artikelnr.: 38185532

Inhaltsangabe

List of contributors; Preface Manuel Marí; Introduction Jennifer Knight;
Part I. Graftings: 1. Two developmental gradients control head formation in
hydra H. R. Bode; 2. Embryonic regulation and induction in sea urchin
development C. A. Ettensohn; 3. The isthmic organizer and brain
regionalization in chick embryos D. Echevaarría and S. Martínez; Part II.
Specific Chemical Reagents: 4. Chemotaxis of aggregating Dictyostelium
cells G. Gerisch and M. Ecke; 5. Inhibition of signal transduction pathways
prevents head regeneration in hydra L. M. Salgado; 6. Retinoic acid during
limb regeneration M. Maden; Part III. Beads Implantation: 7. Experimental
techniques in avian embryos Y. Gañán, J. Rodríguez-León and D. Macías; 8.
Induction of ectopic limb outgrowth in chick with FGF-8 A. Raya, C.
Rodríguez Esteban and J. C. Izpisúa-Belmonte; Part IV. Nucleic Acid
Injections: 9. RNAi techniques applied to freshwater planarians
(Platyhelminthes) during regeneration D. Bueno, R. Romero and E. Saló; 10.
Microinjection of Xenopus embryos R. J. Garriock and P. A. Krieg; Part V.
Genetic Analysis: 11. Segmental specification in Drosophila melanogaster L.
De Navas, M. Suzanne, D. Foronda and E. Sánchez-Herrero; 12. Genetic
analysis of flower development in Arabidopsis thaliana: the ABC model of
floral organ identity determination J. L. Riechmann; 13. Genetic analysis
of vulva development in C. elegans S. Canevascini; Part VI. Clonal
Analysis: 14. The role of the gene apterous in the development of the
Drosophila wing F. J. Díaz-Benjumea; 15. Extramacrochaetae, an example of a
gene required for control of limb size and cell differentiation during wing
morphogenesis in Drosophila A. Baonza; 16. Hedgehog transduction pathway is
involved in pattern formation in Drosophila melanogaster tergites M.
Marí-Beffa; Part VII. In situ Hybridization: 17. Retinoic acid signalling
controls anteroposterior patterning of the zebrafish hindbrain G. Begemann;
18. Left-right asymmetry in the mouse M. Blum, A. Schweickert and C.
Karcher; Part VIII. Transgenic Organisms: 19. Bicoid and Dorsal: two
transcriptions factor gradients which specify cell fates in the early
Drosophila embryo S. Roth; 20. Significance of the temporal modulation of
Hox gene expression on segment morphology J. Castelli-Gair Hombría; 21. The
UAS/GAL4 system for tissue-specific analysis of EGFR gene function in
Drosophila melanogaster J. B. Duffy and N. Perrimon; 22. Neurogenesis in
Drosophila: a genetic approach C. Klämbt and H. Vaessin; 23. Role of the
achaete-scute complex genes in the development of the adult peripheral
nervous system of Drosophila melanogaster S. Sotillos and S. Campuzano;
Part IX. Vertebrate Cloning: 24. The conservation of the genome and nuclear
reprogramming in Xenopus J. B. Gurdon; Part X. Cell Culture: 25. In vitro
culture and differentiation of mouse embryonic stem cells A. Rolletschek,
C. Wiese and A. M. Wobus; Part XI. Evo-Devo Studies: 26. Microevolution
between Drosophila species N. Skaer and P. Simpson; Part XII. Computational
Modelling: 27. Theories as a tool to understand the complex network of
molecular interactions M. Meinhardt; Appendices; Index.