Small Non-Coding RNAs
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This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known…mehr

Produktbeschreibung
This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will be of valuable help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.

  • Produktdetails
  • Methods in Molecular Biology 2300
  • Verlag: Humana / Springer US / Springer, Berlin
  • Artikelnr. des Verlages: 978-1-0716-1385-6
  • 2. Aufl.
  • Seitenzahl: 280
  • Erscheinungstermin: 2. April 2021
  • Englisch
  • Abmessung: 260mm x 183mm x 21mm
  • Gewicht: 729g
  • ISBN-13: 9781071613856
  • ISBN-10: 1071613855
  • Artikelnr.: 60702184
Inhaltsangabe
 PART I INTRODUCTION TO SMALL NON-CODING RNAS 1 Regulatory Non-coding RNAs: An OverviewCamille Virciglio, Yoann Abel, and Mathieu Rederstorff 2 RNA PrecipitationValérie Bourguignon-Igel, Yoann Abel, and Mathieu Rederstorff 3 Quantitative and Qualitative Assessment of Small RNA PreparationsVirginie Marchand, Lilia Ayadi, Valérie Bourguignon-Igel, and Yuri Motorin 4 Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs TransfectionCharbel Alfeghaly, Christelle Aigueperse, Sylvain Maenner, and Isabelle Behm-Ansmant  PART II DETECTION OF SMALL NON-CODING RNAS 5 Northern Blot Detection of Tiny RNAsJana C. Wiegard, M. Amri C. Schlüter, Olga Y. Burenina, Elena A. Kubareva, Gabriele Klug, Arnold Grünweller, and Roland K. Hartmann 6 Stem-loop qRT-PCR Based Quantification of miRNAsYoann Abel and Mathieu Rederstorff 7 Detection and Labeling of Small Non-coding RNAs by Splinted LigationMarlène Vayssières, Gabrielle Bourgeois, Florian Chardon, Anne-Sophie Tillault, and Magali Blaud 8 Fluorescence In Situ Hybridization Of Small Non-coding RNAsValentin Vautrot, Géraud Heckler, Christelle Aigueperse, and Isabelle Behm-Ansmant  PART III FUNCTIONAL ANALYSIS OF SMALL NON-CODING RNAS  9 Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In VivoLayea Baldini and Stéphane Labialle 10 Purification of RiboNucleoProtein Particles by MS2-MBP Affinity ChromatographyAymeric Sanchez and Sylvain Maenner 11 Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP)Charbel Alfeghaly, Isabelle Behm-Ansmant, and Sylvain Maenner 12 Gene Reporter Assays to Study miRNA FunctionYoann Abel and Mathieu Rederstorff 13 CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In VivoBin Yi, Kristina Larter, and Yaguang Xi  PART IV HIGH-THROUGHPUT ANALYSIS OF SMALL NON-CODING RNAS  14 Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAsQuentin Thuillier and Isabelle Behm-Ansmant 15 Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma Virginie Marchand, Adeline Galvanin, and Yuri Motorin 16 Analysis of the RNA and protein complexome by Grad-seqJens Hör and Jörg Vogel 17 µIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAsFarah Bouhedda, Roger Cubi, Stéphanie Baudrey and Michael Ryckelynck  PART V STRUCTURAL APPROACHES  18 RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCTFatima Alghoul, Gilbert Eriani, and Franck Martin 19 Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAsMarie-Eve Chagot, Marc Quinternet, Xavier Manival, and Isabelle Lebars