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In research and industry, many different organisms are established as hosts for production purposes. For recombinant protein production, a wide range of microbial hosts is industrially established. While many optimized strains (chassis) exist that elevate protein production metabolically optimized chassis are rare. To contribute to this gap, in this thesis, the chassis idea is extended to metabolism. The industrially relevant yeast Pichia pastoris was used as model chassis throughout the thesis.