210,99 €
versandkostenfrei*

inkl. MwSt.
Versandfertig in über 4 Wochen
105 °P sammeln
  • Gebundenes Buch

Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy. In RNA Therapeutics: Function, Design, and Delivery , expert contributors from a broad spectrum of scientific backgrounds highlight the roles that messenger RNAs and small RNAs can play in biology and medicine. While covering the five major RNA-based drugs, namely the use of ribozymes to cleave and/or correct mRNA transcript,…mehr

Produktbeschreibung
Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutics as well as a tool for therapy. In RNA Therapeutics: Function, Design, and Delivery, expert contributors from a broad spectrum of scientific backgrounds highlight the roles that messenger RNAs and small RNAs can play in biology and medicine. While covering the five major RNA-based drugs, namely the use of ribozymes to cleave and/or correct mRNA transcript, the use of siRNA for targeted silencing of gene transcripts, the use of aptamers, like short RNA molecules, for neutralizing the protein functions, the use mRNA-transfected DCs to activate immune system against tumor cells, as well as the use of RNA to reprogram T and/or DC cell function, this extensive volume brings together the fields of coding (mRNA) and non-coding RNA such as ribozymes, RNAse P, siRNAs, and miRNAs into one convenient source. Written in the highly successful Methods in Molecular Biology(TM) series format, the cutting-edge protocol chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and practical tips on troubleshooting and avoiding known pitfalls. Also, the book contains several excellent reviews for teaching purposes.

Authoritative and comprehensive, RNA Therapeutics: Function, Design, and Delivery provides key models and tools which will assist researchers in increasing our understanding of RNA functions, modifications, and their involvement in diseases in order to lead to the design of vital new RNA-based therapeutics.

  • Produktdetails
  • Methods in Molecular Biology 629
  • Verlag: Humana / Humana Press / Springer, Berlin
  • Artikelnr. des Verlages: 12451371
  • Edition
  • Seitenzahl: 526
  • Erscheinungstermin: September 2010
  • Englisch
  • Abmessung: 265mm x 187mm x 38mm
  • Gewicht: 1156g
  • ISBN-13: 9781607616566
  • ISBN-10: 1607616564
  • Artikelnr.: 27361225
Inhaltsangabe
1. RNA Modifications: A Mechanism that Modulates Gene ExpressionJohn Karijolich, Athena Kantartzis, and Yi-Tao Yu2. Quantitative Analysis of RNA ModificationsJohn Karijolich, Athena Kantartzis, and Yi-Tao Yu3. Advances in RNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA InterferenceMouldy Sioud4. Progress in siRNA Delivery: A Multifunctional Approach by Polymeric NanoparticlesWeiwei Gao, Zeyu Xiao, Alex Radovic-Moreno, Jinjun Shi, Robert Langer, and Omid C. Farokhzad5. Optimized Protocols for siRNA Delivery into Monocytes and Dendritic CellsAnne Mobergslien and Mouldy Sioud6. Systemic Delivery of Synthetic siRNAsDag R. Sørensen and Mouldy Sioud7. What Are the Key Targeted Delivery Technologies of siRNA Now?Mouldy Sioud8. Using OligoWalk to Identify Efficient siRNA SequencesDavid H. Mathews9. Jet-Injection of Short Hairpin RNA Encoding Vectors into Tumor CellsWolfgang Walther, Ulrike Stein, and Hermann Lage10. Short Hairpin RNA (shRNA): Design, Delivery, and Assessment of Gene KnockdownChris B. Moore, Elizabeth H. Guthrie, Max Tze-Han Huang, and Debra Taxman11. Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1Sheena M. Saayman, Patrick Arbuthnot, and Marc S. Weinberg12. Functional Studies on RNA-Transfected Cell MicroarraysChristina Sæten Fjeldbo, Kristine Misund, Clara-Cecilie Günther, Mette Langaas, Tonje Strømmen Steigedal, Liv Thommesen, Astrid Lægreid, and Torunn Bruland13. Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell MigrationReiko Onuki-Nagasaki, Akira Nagasaki, Kazumi Hakamada, Taro Q.P. Uyeda, Satoshi Fujita, Masato Miyake, and JunMiyake14. Intron-Mediated RNA Interference, Intronic MicroRNAs, and ApplicationsShao-Yao Ying, Chen Pu Chang, and Shi-Lung Lin15. Inhibition of the microRNA Pathway in Zebrafish by siRNAAnders Fjose and Xiao-Feng Zhao16. Profiling of miRNA Expression and Prediction of Target GenesMouldy Sioud17. Small RNA CloningSeungil Ro and Wei Yan18. In situ Hybridization Detection of microRNAsRui Song, Seungil Ro, and Wei Yan19. Detection and Quantitative Analysis of Small RNAs by PCRSeungil Ro and Wei Yan20. In Vivo Reprogramming of Human Telomerase Reverse Transcriptase (hTERT) by Trans-Splicing Ribozyme to Target Tumor CellsSang-Jin Lee, Seong-Wook Lee, Jin-Sook Jeong, and In-Hoo Kim21. Design and Function of Triplex Hairpin RibozymesGuillermo Aquino-Jarquin, Ramiro Rojas-Hernández, and Luis Marat Alvarez-Salas22. Catalytic M1GS RNA as an Antiviral Agent in AnimalsYong Bai, Paul Jay Fannin Rider, and Fenyong Liu23. Using Live Cells to Generate Aptamers for Cancer StudyLing Meng, Kwame Sefah, Dalia Lopez Colon, Hui Chen, Meghan O'Donoghue, Xiangling Xiong, and Weihong Tan24. Primer-Free Aptamer Selection Using a Random DNA LibraryWeihua Pan and Gary A. Clawson25. Development of TLR 7/8 Small RNA AntagonistsMouldy Sioud26. Modulation of Dendritic Cell Maturation and Function by siRNA-Bearing 5'-TriphosphateMouldy Sioud27. Immunotherapy of Cancer with Dendritic Cells Loaded with Tumor Antigens and Activated through mRNA ElectroporationAn M.T. Van Nuffel, Jurgen Corthals, Bart Neyns, Carlo Heirman, Kris Thielemans, and Aude Bonehill28. Non-MHC-Dependent Redirected T Cells Against Tumor CellsHilde Almåsbak, Marianne
Rezensionen
From the reviews:
"Give us an update statement of the present day possible use of RNA to treat several diseases are well presented thanks to a 30 chapters walk in the RNA world. ... Clearly enough, the book is of great interest to the entire scientific community, no matter if basic researchers, teachers or clinicians!" (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)