Synthetic Biology, Part a Methods for Part/Device Characterization and Chassis Engineering Volume 497
Synthetic Biology, Part a Methods for Part/Device Characterization and Chassis Engineering Volume 497
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Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation.
Section I. Measuring and Engineering Central Dogma Processes
Sequence-specificity and Energy Landscapes of DNA-binding Molecules Promoter Reliability in Modular Transcriptional Networks The Analysis of ChIP-seq Data Using DNA Microarrays to Assay Part Function Orthogonal gene expression in Escherichia coli Directed evolution of promoters and tandem gene arrays for customizing RNA synthesis rates and regulation Design and Connection of Robust Genetic Circuits Engineering RNAi circuits From SELEX to cell: Dual selections for synthetic riboswitches Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells De novo design and construction of an inducible gene expression system in mammalian cells BioBuilding: Using banana-scented bacteria to teach synthetic biology Use of Fluorescence Microscopy to Analyze Genetic Circuit Dynamics Microfluidics for synthetic biology: From design to execution Plate-based assays for light-regulated gene expression systems Spatiotemporal control of small GTPases with light using the LOV domain Light Control of Plasma Membrane Recruitment using the Phy-PIF system Synthetic Physiology: Strategies for Adapting Tools from Nature for Genetically-Targeted Control of Fast Biological Processes Metabolic Pathway Flux Enhancement by Synthetic Protein Scaffolding A Synthetic Iterative Pathway for ketoacid elongation Synthetic Biology in Streptomyces bacteria Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio Modification of the Genome of Rhodobacter sphaeroides and Construction of Synthetic Operons Synthetic Biology in Cyanobacteria: Engineering and Analyzing Novel Functions Developing a Synthetic Signal Transduction System in Plants Lentiviral Vectors to Study Stochastic Noise in Gene Expression Kate Franz, Abhyudai Singh, and Leor S. Weinberger
