High-Throughput Metabolomics
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This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in…mehr

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Produktbeschreibung
This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine.
  • Produktdetails
  • Methods in Molecular Biology .1978
  • Verlag: Springer, Berlin; Springer New York; Humana Press
  • Artikelnr. des Verlages: 978-1-4939-9235-5
  • 1st ed. 2019
  • Erscheinungstermin: 2. Juli 2019
  • Englisch
  • Abmessung: 261mm x 181mm x 35mm
  • Gewicht: 1044g
  • ISBN-13: 9781493992355
  • ISBN-10: 149399235X
  • Artikelnr.: 54888830
Inhaltsangabe
Part I: Methods 1. Sample Preparation and Reporting Standards for Metabolomics of Adherent Mammalian Cells Sarah Hayton, Robert D. Trengove, and Garth L. Maker 2. High-Throughput Metabolomics: Isocratic and Gradient Mass Spectrometry-Based Methods Travis Nemkov, Julie A. Reisz, Sarah Gehrke, Kirk C. Hansen, and Angelo D'Alessandro 3. High-Throughput Metabolomics Based on Direct Mass Spectrometry Analysis in Biomedical Research Raúl González-Domínguez, Álvaro González-Domínguez, Carmen Segundo, Mónica Schwarz, Ana Sayago, Rosa María Mateos, Enrique Durán-Guerrero, Alfonso María Lechuga-Sancho, and Ángeles Fernández-Recamales 4. Traveling Wave Ion Mobility Mass Spectrometry: Metabolomics Applications Giuseppe Paglia and Giuseppe Astarita 5. CE-MS for Metabolomics: A Comparison with Other Techniques Joanna Godzien, Antonia Garcia, Angeles López-Gonzalvez, and Coral Barbas Part II: Lipidomics 6. Overview of Lipid Mass Spectrometry and Lipidomics Simona Zarini, Robert M. Barkley, Miguel A. Gijón, and Robert C. Murphy 7. LC-MS/MS-MRM-Based Targeted Metabolomics for Quantitative Analysis of Polyunsaturated Fatty Acids and Oxylipins Xiaoyun Fu, Mikayla Anderson, Yi Wang, and James C. Zimring 8. Untargeted and Semi-Targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics Julie A. Reisz, Connie Zheng, Angelo D'Alessandro, and Travis Nemkov 9. HPLC-MS/MS Methods for Diacylglycerol and Sphingolipid Molecular Species in Skeletal Muscle Kathleen A. Harrison and Bryan C. Bergman Part III: Metabolomics of Animal and Plant Models 10. Quantification of D- and L-2-Hydroxyglutarate in Drosophila melanogaster Tissue Samples Using Gas Chromatography-Mass Spectrometry Hongde Li and Jason M. Tennessen 11. Comprehensive LC-MS-Based Metabolite Fingerprinting Approach for Plant and Fungal-Derived Samples Kirstin Feussner and Ivo Feussner 12. Untargeted Metabolomics of Plant Leaf Tissues Federica Gevi, Giuseppina Fanelli, Lello Zolla, and Sara Rinalducci Part IV: Tracing Experiments and Metabolic Flux Analysis 13. Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling Gretchen L. Seim, Emily C. Britt, and Jing Fan 14. Quantifying Intermediary Metabolism and Lipogenesis in Cultured Mammalian Cells Using Stable Isotope Tracing and Mass Spectrometry Thekla Cordes and Christian M. Metallo 15. Insights into Dynamic Network States Using Metabolomic Data Reihaneh Mostolizadeh, Andreas Dräger, and Neema Jamshidi 16. Analysis of Endothelial Fatty Acid Metabolism Using Tracer Metabolomics Joanna Kalucka, Bart Ghesquière, Sarah-Maria Fendt, and Peter Carmeliet 17. Using Stable Isotope Tracers for Metabolic Pathway Analysis Sara Violante, Tiffany H. Thomas, and Justin R. Cross Part V: Data Processing in Metabolomics 18. Data Processing for GC-MS and LC-MS-Based Untargeted Metabolomics Linxing Yao, Amy M. Sheflin, Corey D. Broeckling, and Jessica E. Prenni 19. El-MAVEN: A Fast, Robust, and User-Friendly Mass Spectrometry Data Processing Engine for Metabolomics Shubhra Agrawal, Sahil Kumar, Raghav Sehgal, Sabu George, Rishabh Gupta, Surbhi Poddar, Abhishek Jha, and Swetabh Pathak 20. Pre-Analytic Considerations for Mass Spectrometry-Based Untargeted Metabolomics Data Dominik Reinhold, Harrison Pielke-Lombardo, Sean Jacobson, Debashis Ghosh, and Katerina Kechris Part VI: Metabolic Measurements with Techniques Orthogonal to Mass Spectrometry 21. Temporal Metabolite, Ion, and Enzyme Activity Profiling Using Fluorescence Microscopy and Genetically-Encoded Biosensors Douglas A. Chapnick, Eric Bunker, Xuedong Liu, and William M. Old 22. Microplate Assays for Spectrophotometric Measurement of Mitochondrial Enzyme Activity Rachel C. Janssen and Kristen E. Boyle 23. Quantitative NMR-Based Metabolomics on T