The Retinoblastoma Protein
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This volume covers the mechanisms of pRb inactivation detailing repressive mechanisms commonly associated to cancer, and representative of the experimentally relevant tests used in the establishment of cancer diagnosis and prognosis. Chapters contain protocols and in-depth discussions for commonly used experimental approaches to assess the status and function of components of the pRb pathway, including pRb itself, in cell lines and biological samples.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of…mehr

Produktbeschreibung
This volume covers the mechanisms of pRb inactivation detailing repressive mechanisms commonly associated to cancer, and representative of the experimentally relevant tests used in the establishment of cancer diagnosis and prognosis. Chapters contain protocols and in-depth discussions for commonly used experimental approaches to assess the status and function of components of the pRb pathway, including pRb itself, in cell lines and biological samples.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, The Retinoblastoma Protein aims to serve as a guide to assist molecular cancer biologists in their search for understanding of the molecular functions of this preeminent tumor suppressor.

  • Produktdetails
  • Methods in Molecular Biology .1726
  • Verlag: Humana Press / Springer New York / Springer, Berlin
  • Artikelnr. des Verlages: 978-1-4939-7564-8
  • 1st ed. 2018
  • Seitenzahl: 212
  • Erscheinungstermin: 22. Februar 2018
  • Englisch
  • Abmessung: 260mm x 183mm x 17mm
  • Gewicht: 582g
  • ISBN-13: 9781493975648
  • ISBN-10: 1493975641
  • Artikelnr.: 49154207
Inhaltsangabe
1. Characterization of RB1 Deletions in Interphase and Metaphase by Molecular Cytogenetics Exemplified in Chronic Lymphatic Leukemia Thomas Liehr 2. Detection of RB1 Gene Copy Number Variations using a Multiplex Ligation-Dependent Probe Amplification Method Nejat Dalay 3. A Fluorescent Quantitative Multiplex PCR Method to Detect Copy Number Changes in the RB1 Gene Chitra Kannabiran 4. Using Methylation-specific PCR to study RB1 Promoter Hypermethylation Thaís M. McCormick and Maria da Glória da C. Carvalho 5. Detection of Aberrant DNA Methylation Patterns in the RB1 Gene Sumadi Lukman Anwar and Ulrich Lehmann 6. Detection of Retinoblastoma Protein Phosphorylation by Immunoblot Analysis Pedro G. Santiago-Cardona, Jaileene Pérez-Morales, and Jonathan González-Flores
7. Immunohistochemical Detection of the Retinoblastoma Protein Charles A. Ishak, Matthew J. Cecchini, Christopher J. Howlett, and Frederick A. Dick 8. Immunohistochemical Detection of Retinoblastoma Protein Phosphorylation in Human Tumor Samples Jaileene Pérez-Morales, Angel Núñez-Marrero, and Pedro G. Santiago-Cardona 9. Detection of CCND1 Locus Amplification by Fluorescence in situ Hybridization Margit Balázs, Viktória Koroknai, István Szász, and Szilvia Ecsedi 10. Detection of CCND1 Gene Copy Number Variations using Multiplex Ligation-dependent Probe Amplification and Fluorescence in situ Hybridization Methods Akishi Ooi and Takeru Oyama 11. Detection of p16 Promoter Hypermethylation by Methylation-specific PCR Javed Hussain Choudhury, Raima Das, Shaheen Laskar, Sharbadeb Kundu, Manish Kumar, Partha Pratim Das, Yashmin Choudhury, Rosy Mondal, and Sankar Kumar Ghosh 12. Immunohistochemical Detection of p16 in Clinical Samples Georgia Karpathiou and Michel Peoc'h  13. Detection of E2F-DNA Complexes Using Chromatin Immunoprecipitation Assays Miyoung Lee, Lorraine J. Gudas, and Harold I. Saavedra 14. Detection of E2F-induced Transcriptional Activity Using a Dual Luciferase Reporter Assay Ainhoa Iglesias-Ara, Nerea Osinalde, and Ana M. Zubiaga 15. Detection of HPV E6/E7 mRNA in Clinical Samples using RNA in situ Hybridization Manishkumar Pandey, Priyanka G. Bhosale, and Manoj B. Mahimkar 16. CRISPR/Cas9 Mediated Knockout of Rb1 in Xenopus tropicalis Thomas Naert and Kris Vleminckx