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Die neuesten Fortschritte in der Proteomik, die zum großen Teil durch die Entwicklungen in der Massenspektronomie vorangetrieben werden, zeigen immer wieder die Komplexität und Vielfalt pathogener Mechanismen in Mikroben als Verursacher und Verstärker infektiöser Erkrankungen. Eine neue Ära in der medizinischen Mikrobiologie erfordert daher einen schnellen Wechsel der heutigen Verfahren hin zu hoch leistungsfähigen Analysesystemen für die Diagnose mikrobieller Pathogene. Dieses Buch deckt die ganze Bandbreite mikrobiologischer Anwendungen der Proteomik und Massenspektronomie ab. In sechs…mehr

Produktbeschreibung
Die neuesten Fortschritte in der Proteomik, die zum großen Teil durch die Entwicklungen in der Massenspektronomie vorangetrieben werden, zeigen immer wieder die Komplexität und Vielfalt pathogener Mechanismen in Mikroben als Verursacher und Verstärker infektiöser Erkrankungen. Eine neue Ära in der medizinischen Mikrobiologie erfordert daher einen schnellen Wechsel der heutigen Verfahren hin zu hoch leistungsfähigen Analysesystemen für die Diagnose mikrobieller Pathogene. Dieses Buch deckt die ganze Bandbreite mikrobiologischer Anwendungen der Proteomik und Massenspektronomie ab. In sechs Kapiteln werden die Abläufe dargestellt, mit denen die meisten mikrobiologischen Labors das Thema angehen: Übergang, Werkzeuge, Vorbereitung, Profiling-by-Pattern, Zielproteine und Datenanalyse.
  • Produktdetails
  • Verlag: Wiley John + Sons / Wiley, John, & Sons, Inc
  • Artikelnr. des Verlages: 1A470681990
  • Seitenzahl: 534
  • Erscheinungstermin: Juli 2010
  • Englisch
  • Abmessung: 251mm x 176mm x 30mm
  • Gewicht: 1172g
  • ISBN-13: 9780470681992
  • ISBN-10: 0470681993
  • Artikelnr.: 29922117
Inhaltsangabe
Preface List of contributors Microbial Characterisation; the Transition from Conventional Methods to Proteomics. 1) CHANGING CONCEPTS IN THE CHARACTERISATION OF MICROBES AND THE INFLUENCE OF MASS SPECTROMETRY Haroun Shah et al 2) MICROBIAL PHYLOGENY AND EVOLUTION BASED ON PROTEIN SEQUENCES (THE CHANGE FROM TARGETED GENES TO PROTEINS) Radhey Gupta 2: PROTEOMICS TOOLS AND BIOMARKER DISCOVERY. 3) OVERVIEW OF THE PROTEOMIC TOOLS AND IT LINKS TO GENOMICS Raju Misra. 4) HIGH THROUGHPUT BIOMARKER DISCOVERY IN MICROORGANISMS Ming Fang Proteomic Strategies for Protein Identification 1. Bottom
up Proteomics 2. Top
down Proteomics Multidimensional Protein Identification Mass Spectrometry Based Targeted Protein Quantification and Biomarker Discovery Selected Reaction Monitoring Conclusions 5) MALDI MASS SPECTROMETRY IMAGING, A NEW FRONTIER IN BIOSTRUCTURAL TECHNIQUES: APPLICATIONS IN BIOMEDICINE Simona Francese and Malcolm R. Clench 3: PROTEIN SAMPLES PREPARATION TECHNIQUES CONVENTIONAL APPROACHES FOR SAMPLE PREPARATION FOR LIQUID CHROMATOGRAPHY AND TWO
DIMENSIONAL GEL ELECTROPHORESIS Vesela Encheva and Robert Parker 7) ISOLATION AND PREPARATION OF SPORE PROTEINS AND SUBSEQUENT CHARACTERISATION BY ELECTROPHORESIS AND MASS SPECTROMETRY Nicola Thorne, Saheer Gharbia and Haroun Shah 8) CHARACTERIZATION OF BACTERIAL MEMBRANE PROTEINS USING A NOVEL COMBINATION OF A LIPID BASED PROTEIN IMMOBILIZATION TECHNIQUE WITH MASS SPECTROMETRY Roger Karlsson, Darren Chooneea, Elisabet Carlsohn, Vesela Encheva and Haroun Shah 9) Wider Protein Detection from Biological Extracts by the Reduction of Dynamic Concentration Range. Luc Guerrier, Egisto Boschetti and Piergiorgi Roghetti 10) 3D
gel electrophoresis
a new development in protein analysis. Robert Ventzki and Josef Stegemann SECTION 4: CHARACTERISATION OF MICROORGANISMS BY PATTERN MATCHING OF MASS SPECTRAL PROFILES AND BIOMARKER APPROACHES REQUIRING MINIMAL SAMPLE PREPARATION. 11) Microbial Disease Biomarkers using ProteinChip Arrays Shea Hamilton, Michael Levin, J. Simon Kroll, Paul R. Langford 12) MALDI
TOF MS and microbial identification: years of experimental development to an established protocol. Wibke Kallow, Marcel Erhard, Haroun N. Shah, Emmanuel Raptakis, Martin Welker. 5: Targeted Molecules and Analysis of Specific Microorganisms. 13) Whole Cell MALDI Mass Spectrometry for the Rapid Characterisation of Bacteria; A Survey of Applications to Major Phyletic Lines in Microbial Kingdom. Ben van Baar Bacillus spp. Staphylococcus spp. Streptococcus spp. Mycobacterium spp. Other Gram
positive bacteria Escherichia coli Gram
negative food
and waterborne pathogen proteobacteria, other than E. Coli Typical sexually transmitted pathogens: Neisseria spp. and Haemophilus spp. Gram
negative biothreat agent bacteria Other Gram
negative bacteria Pathogenic Cyanobacteria Strategies for the identification of biomarkers in whole cell MALDI MS spectra Protein database consideration On
target treatment and analysis Off
target' Analysis and correlation with proteomics studies General consideration of biomarker identification strategies Conclusions and outlook 14) The power of Gel
based proteomics to understand physiology in Bacillus subtilis Haike Antelmann and Michael Hecker Introduction Results 1 Proteomics of protein secretion mechanisms in Bacillus subtilis 2 Definition of proteomic signatures to study cell physiology 3 Proteomics as tool to visualize reversible and irreversible thiol
modifications 4 Proteomics as tool to define regulon structures and targets for non
coding RNAs 5 Acknowledgment 15) Mass Spectrometry in the study of Tularemia Pathogenesis. Jiri Stulik, Juraj Lenco, Jiri Dresler, Jana Klimentova, Lenka Hernychova, Lucie Balonova and Alena Fucikova. References 16) Bacterial Post
Genomics for Vaccine development Giulia Bernardini, Daniela Braconi and Annalisa Santucci Summary comparative genomics transcriptomics proteomics and immmunoproteomics other high
throughput technologies meningococcal vaccines and reverse vaccinology helicobacter pylori vaccines conclusions references 6 Statistical Analysis of 2D Gels and Analysis of Mass Spectral Data * Machine Learning Techniques for the Analysis of Mass spectrometry Data. Graham Ball and Ali Al
Shahib 18) Mass Spectrometry for microbial Proteomics: Issues in data analysis with electrophoretic or mass spectrometric expression proteomic data. Natasha A. Karp Title page Foreword Section 7: DNA Resequencing by MALDI
TOF
Mass Spectrometry and its Application to Traditional Microbiological Problems. (19) Comparative DNA sequence analysis and typing using Mass Spectrometry Christiane Honisch,Yong Chen and Franz Hillenkamp References (20) Transfer of a Traditional Serotyping System (Kauffmann
White) onto a MALDI
TOF
MS platform for the rapid Typing of Salmonella isolates. Chloe Bishop, Cath Arnold and Saheer Gharbia Typing of salmonella isolates Biology Pathogenesis Clinical Disease Phylogeny Virulence and Gene Transfer Necessity to subtype >1.4 Antigenic Analysis
The Traditional Kauffmann
White Schema and its future Serotyping Flagellar Antigens Flagellar Variation Somatic Antigens Flagellin sequences correspond directly to Salmonella serotype. Specific SNPs Subtyping by antigen sequence Variation of the Rfb Genes Intro Different methods available MALDI
TOF data analysis Salmonella molecular serotyping as a Case Study Gene Selection Results Overview Clustering and Sequence Variation of Amplicons Closing Remarks