Mass Spectrometry for Microbial Proteomics (eBook, ePUB)

• Format: ePub

Produktbeschreibung

New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens. This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject -Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.

Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 534
• Erscheinungstermin: 22. Oktober 2010
• Englisch
• ISBN-13: 9781119991922
• Artikelnr.: 37344563

Autorenporträt

Professor Haroun N. Shah is Head of Molecular Identification Services Unit at the Centre for Infections, Health Protection Agency, London. The Centre for Infections provides infectious disease surveillance and microbial identification services, co-ordinating the investigation and cause of national and uncommon outbreaks of diseases. Haroun holds several chairs at various universities and spent 25 years in academic life at the University of London. Professor Saheer E. Gharbia is Head of the Applied and Functional Genomics Unit at the HPA Centre for Infections. She has a PhD in molecular Genetics and Biochemistry, and has held various academic positions at the University of London and McGill University, Canada.

Inhaltsangabe

Preface List of contributors Microbial Characterisation; the Transition
from Conventional Methods to Proteomics. 1) CHANGING CONCEPTS IN THE
CHARACTERISATION OF MICROBES AND THE INFLUENCE OF MASS SPECTROMETRY Haroun
Shah et al 2) MICROBIAL PHYLOGENY AND EVOLUTION BASED ON PROTEIN SEQUENCES
(THE CHANGE FROM TARGETED GENES TO PROTEINS) Radhey Gupta 2: PROTEOMICS
TOOLS AND BIOMARKER DISCOVERY. 3) OVERVIEW OF THE PROTEOMIC TOOLS AND IT
LINKS TO GENOMICS Raju Misra. 4) HIGH THROUGHPUT BIOMARKER DISCOVERY IN
MICROORGANISMS Ming Fang Proteomic Strategies for Protein Identification 1.
Bottom-up Proteomics 2. Top-down Proteomics Multidimensional Protein
Identification Mass Spectrometry Based Targeted Protein Quantification and
Biomarker Discovery Selected Reaction Monitoring Conclusions 5) MALDI MASS
SPECTROMETRY IMAGING, A NEW FRONTIER IN BIOSTRUCTURAL TECHNIQUES:
APPLICATIONS IN BIOMEDICINE Simona Francese and Malcolm R. Clench 3:
PROTEIN SAMPLES PREPARATION TECHNIQUES CONVENTIONAL APPROACHES FOR SAMPLE
PREPARATION FOR LIQUID CHROMATOGRAPHY AND TWO-DIMENSIONAL GEL
ELECTROPHORESIS Vesela Encheva and Robert Parker 7) ISOLATION AND
PREPARATION OF SPORE PROTEINS AND SUBSEQUENT CHARACTERISATION BY
ELECTROPHORESIS AND MASS SPECTROMETRY Nicola Thorne, Saheer Gharbia and
Haroun Shah 8) CHARACTERIZATION OF BACTERIAL MEMBRANE PROTEINS USING A
NOVEL COMBINATION OF A LIPID BASED PROTEIN IMMOBILIZATION TECHNIQUE WITH
MASS SPECTROMETRY Roger Karlsson, Darren Chooneea, Elisabet Carlsohn,
Vesela Encheva and Haroun Shah 9) Wider Protein Detection from Biological
Extracts by the Reduction of Dynamic Concentration Range. Luc Guerrier,
Egisto Boschetti and Piergiorgi Roghetti 10) 3D-gel electrophoresis - a new
development in protein analysis. Robert Ventzki and Josef Stegemann SECTION
4: CHARACTERISATION OF MICROORGANISMS BY PATTERN MATCHING OF MASS SPECTRAL
PROFILES AND BIOMARKER APPROACHES REQUIRING MINIMAL SAMPLE PREPARATION. 11)
Microbial Disease Biomarkers using ProteinChip Arrays Shea Hamilton,
Michael Levin, J. Simon Kroll, Paul R. Langford 12) MALDI-TOF MS and
microbial identification: years of experimental development to an
established protocol. Wibke Kallow, Marcel Erhard, Haroun N. Shah, Emmanuel
Raptakis, Martin Welker. 5: Targeted Molecules and Analysis of Specific
Microorganisms. 13) Whole Cell MALDI Mass Spectrometry for the Rapid
Characterisation of Bacteria; A Survey of Applications to Major Phyletic
Lines in Microbial Kingdom. Ben van Baar Bacillus spp. Staphylococcus spp.
Streptococcus spp. Mycobacterium spp. Other Gram-positive bacteria
Escherichia coli Gram-negative food- and waterborne pathogen
proteobacteria, other than E. Coli Typical sexually transmitted pathogens:
Neisseria spp. and Haemophilus spp. Gram-negative biothreat agent bacteria
Other Gram-negative bacteria Pathogenic Cyanobacteria Strategies for the
identification of biomarkers in whole cell MALDI MS spectra Protein
database consideration On-target treatment and analysis Off-target'
Analysis and correlation with proteomics studies General consideration of
biomarker identification strategies Conclusions and outlook 14) The power
of Gel-based proteomics to understand physiology in Bacillus subtilis Haike
Antelmann and Michael Hecker Introduction Results 1 Proteomics of protein
secretion mechanisms in Bacillus subtilis 2 Definition of proteomic
signatures to study cell physiology 3 Proteomics as tool to visualize
reversible and irreversible thiol- modifications 4 Proteomics as tool to
define regulon structures and targets for non- coding RNAs 5 Acknowledgment
15) Mass Spectrometry in the study of Tularemia Pathogenesis. Jiri Stulik,
Juraj Lenco, Jiri Dresler, Jana Klimentova, Lenka Hernychova, Lucie
Balonova and Alena Fucikova. References 16) Bacterial Post-Genomics for
Vaccine development Giulia Bernardini, Daniela Braconi and Annalisa
Santucci Summary comparative genomics transcriptomics proteomics and
immmunoproteomics other high-throughput technologies meningococcal vaccines
and reverse vaccinology helicobacter pylori vaccines conclusions references
6 Statistical Analysis of 2D Gels and Analysis of Mass Spectral Data *
Machine Learning Techniques for the Analysis of Mass spectrometry Data.
Graham Ball and Ali Al-Shahib 18) Mass Spectrometry for microbial
Proteomics: Issues in data analysis with electrophoretic or mass
spectrometric expression proteomic data. Natasha A. Karp Title page
Foreword Section 7: DNA Resequencing by MALDI-TOF-Mass Spectrometry and its
Application to Traditional Microbiological Problems. (19) Comparative DNA
sequence analysis and typing using Mass Spectrometry Christiane
Honisch,Yong Chen and Franz Hillenkamp References (20) Transfer of a
Traditional Serotyping System (Kauffmann-White) onto a MALDI-TOF-MS
platform for the rapid Typing of Salmonella isolates. Chloe Bishop, Cath
Arnold and Saheer Gharbia Typing of salmonella isolates Biology
Pathogenesis Clinical Disease Phylogeny Virulence and Gene Transfer
Necessity to subtype >1.4 Antigenic Analysis - The Traditional Kauffmann -
White Schema and its future Serotyping Flagellar Antigens Flagellar
Variation Somatic Antigens Flagellin sequences correspond directly to
Salmonella serotype. Specific SNPs Subtyping by antigen sequence Variation
of the Rfb Genes Intro Different methods available MALDI-TOF data analysis
Salmonella molecular serotyping as a Case Study Gene Selection Results
Overview Clustering and Sequence Variation of Amplicons Closing Remarks