Aptamers in Bioanalysis (eBook, PDF)
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This is the first book to detail bioanalytical technologies and methods that have been developed using aptamers in analytical, medical, environmental, and food science applications. After an introduction to aptamers, aptamer targets, and their general uses, it discusses different applications with particular attention to the comparison between aptamer-based biosensors and methods versus the corresponding immunosensors. Examples of aptamer-based diagnostic techniques include whole-cell protein profiling (proteomics) and medical diagnostics for the distinction of diseased versus healthy states.…mehr
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- Produktdetails
- Verlag: John Wiley & Sons
- Seitenzahl: 336
- Erscheinungstermin: 28. April 2009
- Englisch
- ISBN-13: 9780470380765
- Artikelnr.: 37291917
- Verlag: John Wiley & Sons
- Seitenzahl: 336
- Erscheinungstermin: 28. April 2009
- Englisch
- ISBN-13: 9780470380765
- Artikelnr.: 37291917
(Jean-Jacques Toulme , Jean-Pierre Daguer, and Eric Dausse). 1.1
Introduction. 1.2 The Power of Selection and Aptamer Refinement. 1.3 The
Chemistry Drives the Shape. 1.4 Aptaregulators. 1.5 Aptasensors. 1.6
Prospects. References. 2 SELEX AND ITS RECENT OPTIMIZATIONS (Beate
Strehlitz and Regina Stoltenburg). 2.1 Introduction. 2.2 Aptamers and Their
Selection by SELEX. 2.3 Modifications of SELEX Technology. 2.4 Advantages
and Limitations of Aptamers and Their Selection Technology. 2.5
Applications of Aptamers Being Developed for the Market. 2.6 Future
Perspectives. References. II BIOSENSORS. 3 ELECTROCHEMICAL APTASENSORS
(Itamar Willner and Maya Zayats). 3.1 Introduction. 3.2 Electrochemical
Aptasensor Based on Redox-Active Aptamer Monolayers Linked to Electrodes.
3.3 Enzyme-Based Amplified Electrochemical Aptasensors. 3.4 Amplified
Electrochemical Aptasensors Based on Nanoparticles. 3.5 Label-Free
Electrochemical Aptasensors. 3.6 Field-Effect Transistor-Based Aptasensors.
3.7 Conclusions and Perspectives. References. 4 APTAMERS: HYBRIDS BETWEEN
NATURE AND TECHNOLOGY (Moritz K. Beissenhirtz, Eik Leupold, Walter
Stocklein, Ulla Wollenberger, Oliver Panke, Fred Lisdat, and Frieder W.
Scheller). 4.1 Introduction. 4.2 Specific Features of Aptamers. 4.3
Electrochemical Detection of Nucleic Acids. 4.4 Cytochrome c Binding by
Aptamers. 4.5 DNA Machines and Aptamers. References. 5 DETECTION OF
PROTEIN-APTAMER INTERACTIONS BY MEANS OF ELECTROCHEMICAL INDICATORS AND
TRANSVERSE SHEAR MODE METHOD (Tibor Hianik). 5.1 Introduction. 5.2
Immobilization of Aptamers on a Solid Support. 5.3 Detection of
Aptamer-Ligand Interactions. 5.3.1 Electrochemical Methods. 5.3.2 Acoustic
Methods. 5.4 Conclusions. References. 6 BIOSENSORS USING THE APTAMERIC
ENZYME SUBUNIT: THE USE OF APTAMERS IN THE ALLOSTERIC CONTROL OF ENZYMES
(Kazunori Ikebukuro, Wataru Yoshida, and Koji Sode). 6.1 Aptamers as
Molecular Recognition Elements of Biosensors. 6.1.1 Comparing Aptamers to
Antibodies. 6.1.2 Signaling Aptamers. 6.2 Homogeneous Sensing. 6.2.1
Biosensor Systems That Do Not Require Bound-Free Separation. 6.2.2
Aptameric Enzyme Subunit. 6.3 Evolution-mimicking Algorithm for the
Improvement of Aptamers. References. 7 NANOMATERIAL-BASED LABEL-FREE
APTASENSORS (Kagan Kerman and Eiichi Tamiya). 7.1 Introduction. 7.2
Label-Free Electrochemical Aptasensors. 7.3 Field-Effect Transistor-Based
Aptasensors. 7.4 Label-Free Aptasensors Based on Localized Surface Plasmon
Resonance. 7.5 Forthcoming Challenges and Concluding Remarks. References. 8
APTAMER-BASED BIOANALYTICAL ASSAYS: AMPLIFICATION STRATEGIES (Sara
Tombelli, Maria Minunni, and Marco Mascini). 8.1 Introduction. 8.2
Bioanalytical Assays Based on Aptamer-Functionalized Nanoparticles. 8.3
Aptamers and Quantum Dot-Based Assays. 8.4 Aptazymes and Aptamer-Based
Machines. 8.5 Polymerase Chain Reaction as an Amplification Method in
Aptamer-Based Assays. 8.6 Conclusions. References. III APPLICATIONS. 9
KINETIC CAPILLARY ELECTROPHORESIS FOR SELECTION, CHARACTERIZATION, AND
ANALYTICAL UTILIZATION OF APTAMERS (Sergey N. Krylov). 9.1 Introduction.
9.1.1 Kinetic Capillary Electrophoresis. 9.1.2 The Concept of NECEEM and
ECEEM. 9.2 Selection of Aptamers Using KCE Methods for Partitioning and
Affinity Control. 9.2.1 NECEEM-Based Selection of Aptamers. 9.2.2
ECEEM-Based Selection of Aptamers. 9.2.3 Optimization of PCR. 9.2.4 Future
of KCE Methods for Aptamer Selection. 9.3 Measurements of Binding
Parameters of Target-Aptamer Interaction by KCE Methods. 9.3.1 Foundation.
9.3.2 Temperature Control Inside the Capillary. 9.3.3 Examples. 9.4
Quantitative Affinity Analysis of a Target Using Aptamer as an Affinity
Probe. 9.4.1 Foundation. 9.4.2 Example. 9.5 Conclusions. References. 10
APTAMERS FOR SEPARATION OF ENANTIOMERS (Corinne Ravelet and Eric Peyrin).
10.1 Introduction. 10.2 Generation and Properties of Enantioselective
Aptamers. 10.3 Immobilized Aptamers for Enantiomeric Separation by Liquid
Chromatography. 10.3.1 Stationary-Phase Preparation and Column Packing.
10.3.2 DNA Aptamer-Based CSPs. 10.3.3 RNA Aptamer-Based CSPs and the
Mirror-Image Strategy. 10.3.4 Class-Specific Aptamer-Based CSPs. 10.4
Aptamers for Analysis of Enantiomers by Capillary Electrophoresis. 10.4.1
Aptamers as Chiral Additives in the Background Electrolyte for CE
Enantiomeric Separation. 10.4.2 Aptamers for the Design of an Affinity
CE-Based Enantioselective Competitive Assay. 10.5 Conclusions. References.
11 APTAMER-MODIFIED SURFACES FOR AFFINITY CAPTURE AND DETECTION OF PROTEINS
IN CAPILLARY ELECTROPHORESIS AND MALDI-MASS SPECTROMETRY (Linda B. McGown).
11.1 Introduction. 11.2 Aptamer-Modified Capillaries in Affinity Capillary
Electrophoresis. 11.3 Aptamer-Modified Surfaces for Affinity MALDI-MS.
11.3.1 Overview. 11.3.2 Affinity MALDI-MS of Thrombin. 11.3.3 Affinity
MALDI-MS of IgE. 11.3.4 Summary. 11.4 Beyond Aptamers: Genome-Inspired DNA
Binding Ligands. References. 12 STRATEGY FOR USE OF SMART ROUTES TO PREPARE
LABEL-FREE APTASENSORS FOR BIOASSAY USING DIFFERENT TECHNIQUES (Bingling
Li, Hui Wei, and Shaojun Dong). 12.1 Introduction. 12.2 Electrochemical
Aptasensors. 12.2.1 POSOALF Mode. 12.2.2 PFSOALF Mode. 12.2.3
Electrochemical Impedimetric Aptasensors. 12.2.4 Electrochemical
Aptasensors with Nonlabeled Redox Probes. 12.3 Fluorescent Molecular
Switches. 12.3.1 POSFALF Mode. 12.3.2 PFSFALF Mode. 12.4 Colorimetry.
12.4.1 POSFALF Mode. 12.4.2 PFSFALF Mode. 12.5 Hemin-Aptamer DNAzyme-Based
Aptasensor. 12.6 Liquid Chromatography, Electrochromatography, and
Capillary Electrophoresis Applications. 12.7 Other Aptasensors. 12.8
Conclusions. References. INDEX.
(Jean-Jacques Toulme , Jean-Pierre Daguer, and Eric Dausse). 1.1
Introduction. 1.2 The Power of Selection and Aptamer Refinement. 1.3 The
Chemistry Drives the Shape. 1.4 Aptaregulators. 1.5 Aptasensors. 1.6
Prospects. References. 2 SELEX AND ITS RECENT OPTIMIZATIONS (Beate
Strehlitz and Regina Stoltenburg). 2.1 Introduction. 2.2 Aptamers and Their
Selection by SELEX. 2.3 Modifications of SELEX Technology. 2.4 Advantages
and Limitations of Aptamers and Their Selection Technology. 2.5
Applications of Aptamers Being Developed for the Market. 2.6 Future
Perspectives. References. II BIOSENSORS. 3 ELECTROCHEMICAL APTASENSORS
(Itamar Willner and Maya Zayats). 3.1 Introduction. 3.2 Electrochemical
Aptasensor Based on Redox-Active Aptamer Monolayers Linked to Electrodes.
3.3 Enzyme-Based Amplified Electrochemical Aptasensors. 3.4 Amplified
Electrochemical Aptasensors Based on Nanoparticles. 3.5 Label-Free
Electrochemical Aptasensors. 3.6 Field-Effect Transistor-Based Aptasensors.
3.7 Conclusions and Perspectives. References. 4 APTAMERS: HYBRIDS BETWEEN
NATURE AND TECHNOLOGY (Moritz K. Beissenhirtz, Eik Leupold, Walter
Stocklein, Ulla Wollenberger, Oliver Panke, Fred Lisdat, and Frieder W.
Scheller). 4.1 Introduction. 4.2 Specific Features of Aptamers. 4.3
Electrochemical Detection of Nucleic Acids. 4.4 Cytochrome c Binding by
Aptamers. 4.5 DNA Machines and Aptamers. References. 5 DETECTION OF
PROTEIN-APTAMER INTERACTIONS BY MEANS OF ELECTROCHEMICAL INDICATORS AND
TRANSVERSE SHEAR MODE METHOD (Tibor Hianik). 5.1 Introduction. 5.2
Immobilization of Aptamers on a Solid Support. 5.3 Detection of
Aptamer-Ligand Interactions. 5.3.1 Electrochemical Methods. 5.3.2 Acoustic
Methods. 5.4 Conclusions. References. 6 BIOSENSORS USING THE APTAMERIC
ENZYME SUBUNIT: THE USE OF APTAMERS IN THE ALLOSTERIC CONTROL OF ENZYMES
(Kazunori Ikebukuro, Wataru Yoshida, and Koji Sode). 6.1 Aptamers as
Molecular Recognition Elements of Biosensors. 6.1.1 Comparing Aptamers to
Antibodies. 6.1.2 Signaling Aptamers. 6.2 Homogeneous Sensing. 6.2.1
Biosensor Systems That Do Not Require Bound-Free Separation. 6.2.2
Aptameric Enzyme Subunit. 6.3 Evolution-mimicking Algorithm for the
Improvement of Aptamers. References. 7 NANOMATERIAL-BASED LABEL-FREE
APTASENSORS (Kagan Kerman and Eiichi Tamiya). 7.1 Introduction. 7.2
Label-Free Electrochemical Aptasensors. 7.3 Field-Effect Transistor-Based
Aptasensors. 7.4 Label-Free Aptasensors Based on Localized Surface Plasmon
Resonance. 7.5 Forthcoming Challenges and Concluding Remarks. References. 8
APTAMER-BASED BIOANALYTICAL ASSAYS: AMPLIFICATION STRATEGIES (Sara
Tombelli, Maria Minunni, and Marco Mascini). 8.1 Introduction. 8.2
Bioanalytical Assays Based on Aptamer-Functionalized Nanoparticles. 8.3
Aptamers and Quantum Dot-Based Assays. 8.4 Aptazymes and Aptamer-Based
Machines. 8.5 Polymerase Chain Reaction as an Amplification Method in
Aptamer-Based Assays. 8.6 Conclusions. References. III APPLICATIONS. 9
KINETIC CAPILLARY ELECTROPHORESIS FOR SELECTION, CHARACTERIZATION, AND
ANALYTICAL UTILIZATION OF APTAMERS (Sergey N. Krylov). 9.1 Introduction.
9.1.1 Kinetic Capillary Electrophoresis. 9.1.2 The Concept of NECEEM and
ECEEM. 9.2 Selection of Aptamers Using KCE Methods for Partitioning and
Affinity Control. 9.2.1 NECEEM-Based Selection of Aptamers. 9.2.2
ECEEM-Based Selection of Aptamers. 9.2.3 Optimization of PCR. 9.2.4 Future
of KCE Methods for Aptamer Selection. 9.3 Measurements of Binding
Parameters of Target-Aptamer Interaction by KCE Methods. 9.3.1 Foundation.
9.3.2 Temperature Control Inside the Capillary. 9.3.3 Examples. 9.4
Quantitative Affinity Analysis of a Target Using Aptamer as an Affinity
Probe. 9.4.1 Foundation. 9.4.2 Example. 9.5 Conclusions. References. 10
APTAMERS FOR SEPARATION OF ENANTIOMERS (Corinne Ravelet and Eric Peyrin).
10.1 Introduction. 10.2 Generation and Properties of Enantioselective
Aptamers. 10.3 Immobilized Aptamers for Enantiomeric Separation by Liquid
Chromatography. 10.3.1 Stationary-Phase Preparation and Column Packing.
10.3.2 DNA Aptamer-Based CSPs. 10.3.3 RNA Aptamer-Based CSPs and the
Mirror-Image Strategy. 10.3.4 Class-Specific Aptamer-Based CSPs. 10.4
Aptamers for Analysis of Enantiomers by Capillary Electrophoresis. 10.4.1
Aptamers as Chiral Additives in the Background Electrolyte for CE
Enantiomeric Separation. 10.4.2 Aptamers for the Design of an Affinity
CE-Based Enantioselective Competitive Assay. 10.5 Conclusions. References.
11 APTAMER-MODIFIED SURFACES FOR AFFINITY CAPTURE AND DETECTION OF PROTEINS
IN CAPILLARY ELECTROPHORESIS AND MALDI-MASS SPECTROMETRY (Linda B. McGown).
11.1 Introduction. 11.2 Aptamer-Modified Capillaries in Affinity Capillary
Electrophoresis. 11.3 Aptamer-Modified Surfaces for Affinity MALDI-MS.
11.3.1 Overview. 11.3.2 Affinity MALDI-MS of Thrombin. 11.3.3 Affinity
MALDI-MS of IgE. 11.3.4 Summary. 11.4 Beyond Aptamers: Genome-Inspired DNA
Binding Ligands. References. 12 STRATEGY FOR USE OF SMART ROUTES TO PREPARE
LABEL-FREE APTASENSORS FOR BIOASSAY USING DIFFERENT TECHNIQUES (Bingling
Li, Hui Wei, and Shaojun Dong). 12.1 Introduction. 12.2 Electrochemical
Aptasensors. 12.2.1 POSOALF Mode. 12.2.2 PFSOALF Mode. 12.2.3
Electrochemical Impedimetric Aptasensors. 12.2.4 Electrochemical
Aptasensors with Nonlabeled Redox Probes. 12.3 Fluorescent Molecular
Switches. 12.3.1 POSFALF Mode. 12.3.2 PFSFALF Mode. 12.4 Colorimetry.
12.4.1 POSFALF Mode. 12.4.2 PFSFALF Mode. 12.5 Hemin-Aptamer DNAzyme-Based
Aptasensor. 12.6 Liquid Chromatography, Electrochromatography, and
Capillary Electrophoresis Applications. 12.7 Other Aptasensors. 12.8
Conclusions. References. INDEX.