G-Quadruplex DNA - Baumann, Peter (Hrsg.)
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Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In "G-Quadruplex DNA: Methods and Protocols", experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular…mehr

Produktbeschreibung
Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In "G-Quadruplex DNA: Methods and Protocols", experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, "G-Quadruplex DNA: Methods and Protocols "promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.

  • Produktdetails
  • Methods in Molecular Biology 608
  • Verlag: Humana / Humana Press / Springer, Berlin
  • Artikelnr. des Verlages: 11995883
  • 2010
  • Seitenzahl: 288
  • Erscheinungstermin: 18. Dezember 2009
  • Englisch
  • Abmessung: 260mm x 183mm x 22mm
  • Gewicht: 804g
  • ISBN-13: 9781588299505
  • ISBN-10: 1588299503
  • Artikelnr.: 27020702
Autorenporträt
Peter Baumann ist Rechtsanwalt in München mit den Schwerpunkten Arbeitsrecht und Scheidungsrecht.
Inhaltsangabe
1. G-Quadruplexes: From Guanine Gels to ChemotherapeuticsTracy M. Bryan and Peter Baumann2. Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand ComplexesShozeb Haider and Stephen Neidle3. Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming PotentialPaul Ryvkin, Steve G. Hershman, Li-San Wang, and F. Brad Johnson4. Preparation of G-Quartet Structures and Detection by Native Gel ElectrophoresisIan K. Moon and Michael B. Jarstfer5. Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop AssayDaekyu Sun and Laurence H. Hurley6. Real-time Observation of G-Quadruplex Dynamics Using Single Molecule FRET MicroscopyBurak Okumus and Taekjip Ha7. Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex StructuresNichola C. Garbett, Chongkham S. Mekmaysy, and Jonathan B. Chaires8. 2-Aminopurine as a Probe for Quadruplex Loop StructuresRobert D. Gray, Luigi Pettracone, Robert Buscaglia, and Jonathan B. Chaires9. Assessing DNA Structures with 125I RadioprobingTimur I. Gaynutdinov, Ronald D. Neumann, and Igor G. Panyutin10. Monitoring the Temperature Unfolding of G-Quadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry TechniquesChris M. Olsen and Luis A. Marky11. Probing Telomeric G-Quadruplex DNA Structures in Cells with in vitro Generated Single-Chain Antibody FragmentsChristiane Schaffitzel, Jan Postberg, Katrin Paeschke, and Hans J. Lipps12. Detection of G-Quadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVCTa-Chau Chang and Cheng-Chung Chang13. Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity ChromatographyJasmine S. Smith and F. Brad Johnson14. Quantifying Interactions between G-Quadruplex DNA and Transition Metal ComplexesRoxanne Kieltyka, Pablo Englebienne, Nicolas Moitessier, and Hanadi Sleiman15. G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex LigandsDavid Monchaud and Marie-Paule Teulade-Fichou
Rezensionen
From the reviews:
"The book G-Quadruplex DNA ... provides an excellent collection of important methods for the study of the DNA G-quadruplex structures. ... I definitely recommend this book, which can be considered an outstanding source of information that addresses several problems ... in the investigation of G-quadruplex structures. The book is appropriate for everyone working in the area of G-quadruplex research, including biologists, biochemists and chemists since it is a worthy guide for both beginners and experienced scientists interested in studying this increasingly attractive field." (Aldo Galeone, ChemBioChem, Issue 11, September, 2010)