Wolfgang Schumann, Dusko Ehrlich, N. Ogasawara

Functional Analysis of Bacterial Genes

A Practical Manual
Herausgegeben:Schumann, Wolfgang; Ehrlich, S. Dusko; Ogasawara, Naotake

• Broschiertes Buch

Produktbeschreibung

Functional analysis is the phase of molecular genetics after the sequencing of a genome, to discover the function of the genes that have been found. This is the first publication of the model bacterial system for the new and important area of functional analysis. The material explains how to carry out functional analysis in bacteria and offers an increased understanding of the biology of Bacillus subtilis.

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Functional Analysis of Bacterial Genes: A Practical Manual summarises the principles and the key methods used to analyse the function of genes in bacteria. Written by members of the Bacillus subtilis Functional Analysis Consortium, this book provides a guide to the many approaches for the systematic analysis of gene function.
Features include:
_ In-depth discussion of the theory and application of the methods
_ A step-by-step practical guide to each method
_ Experimental examples to indicate expected results
_ Contact information for suppliers and resource centres
Written by internationally recognised authors, Functional Analysis of Bacterial Genes: A Practical Manual will be a valuable resource to researchers and technicians using functional analysis for many bacterial species, whether in the academic departments of microbiology or genetics, the biotechnology, biomedicine or pharmaceutical industries, or other centres of genomics and bioinformatics.

"This volume, and the pioneering effort it represents, will be of interest not only to those of us who are specialists in B. subtilis but to all microbiologists and genome scientists who face the formidable task of uncovering the functions of the myriad uncharacterised genes that are emerging from large-scale sequencing." - Richard Losick , The Biological Laboratories, Harvard University, Cambridge, Massachusetts, USA

Produktdetails

• Verlag: Wiley & Sons
• Artikelnr. des Verlages: 14649008000
• 1. Auflage
• Seitenzahl: 404
• Erscheinungstermin: 8. Februar 2001
• Deutsch, Englisch
• Abmessung: 244mm x 170mm x 22mm
• Gewicht: 686g
• ISBN-13: 9780471490081
• ISBN-10: 0471490083
• Artikelnr.: 09462052

Autorenporträt

Wolfgang Schumann, Institute of Genetics, University of Bayreuth, Germany S. Dusko Erhlich, Laboratoire de Génétique Microbienne, INRA, Jouy-en-Josas, France Naotake Ogasawara, Graduate School of Biological Sciences, NAIST, Ikoma, Japan

Inhaltsangabe

List of Contributors. Foreword. Preface. Safety. Aims of the Functional
Analysis Project. In silico Genome Analysis. Inactivation of Bacillus
subtilis Genes Without Known Functions. Analysis of Essential Genes.
Transcriptional Analysis of Large Regions of the Bacterial Genome. Useful
Methods for Transcriptional Analysis. Northern Blot Analysis with non-RI
Detection Systems. Micado, an Integrative Database Dedicated to the
Functional Analysis of Bacillus subtilis and Microbial Genomics. Phenotype
Responses and Reporter Gene Activity from the Systematic Functional
Analysis of Bacillus subtilis Unknown Genes. The Contribution of the EC
Consortium to the Two-dimensional Protein Index of Bacillus subtilis.
Whole-Cell Fingerprinting - Pyrolysis Mass Spectrometry. Metabolism of
Small Molecules and Inorganics. Screening for Genes Involved in Nucleotide
and Nitrogen Metabolism. Screening for Growth and lacZ Reporter Gene
Induction with Metal Ions and Chelators, Amino Acid Analogs, Antibiotics,
Inhibitors of Oxidative Phosphorylation and Miscellaneous Stress Factors.
Nucleotide Metabolism. Utilization of Small Carbon-containing Molecules by
Bacillus subtilis (S. Fillinger, et al.) . Screening for Genes Involved in
the Metabolism of Small Carbon-containing Molecules. Nitrogen Sources.
Induction or Repression by Nitrogen Sources. Glucose Regulation in Bacillus
subtilis. Catabolite Repression by Glucose. Cell Processes and
Macromolecule Metabolism: a Quest for Genes Involved in Genetic Competence,
and DNA Recombination and Repair. Cell Processes and Macromolecule
Metabolism: Methods for the Selection of Mutants in Genetic Competence, DNA
Recombination and Repair and Protein Secretion. Molecular Tools for the
Identification of Mutations Affecting Protein Secretion. Screening for
Mutants Defective in Secretion. Genes Affecting Motility. Screening for
Motility. Differentiation of Bacillus subtilis (A. Moir, et al.) . Methods
for Rapid Screening of Properties: Spore Resistance and Germination. Genes
Affecting the Cell Envelope. Analysis of Cell Envelope Components.
Peptidogylcan Structure and Structural Determination in Vegetative Cells of
Bacillus subtilis (A. Atrih, et al.) . Cell Cycle Gene Analysis.
Identification and Analysis of Cell Cycle Genes. Endospore Formation.
Isolation and Characterization of Genes Affecting Sporulation or the Cell
Cycle. Selection and Characterization of Genes Affecting Germination. The
Oxidative Stress Response. Screening for Increased Sensitivity or
Resistance and Level of lacZ Reporter Gene Expression in Response to the
Oxidizing Agents Hydrogen Peroxide and Methyl Viologen. The Osmotic Shock
Response. Salt Stress. Phosphate Regulation. Screening for Mutants Affected
in their Response to Phosphate. The Heat Shock Response. The Cold Shock
Response. pH-Inducible Genes. Analysis of the Mutant Collection for Defects
in their Temperature and pH Shock Response. Screening for Growth and lacZ
Reporter Gene Induction or Repression under Glucose and Phosphate
Starvation and under Heat, Oxidative and Osmotic Stress. List of Bacillus
subtilis Unknown Genes with a Phenotype Determined by the Functional
Analysis Project. List of Bacillus subtilis Genes of Known Function.
Appendix 1: List of Bacillus subtilis Mutants Constructed by the European
Functional Analysis Project. Appendix 2: List of Bacillys subtilis Mutants
Constructed by the Japanese Functional Analysis Project. Appendix 3: List
of Selected Suppliers. Appendix 4: Glossary. Index.

Rezensionen

"The findings are brought together in this fine book, which may serve in the future as a model for similar publications on other cell types." (Microbiology Today, November 2001)
"Cet ouvrage est donc une tres bonne introduction a l'analyse du genome d'une bacterie." (Sciences Des Aliments - French, No.6, 2001)
"a good practical manual" (Biochemie, No.83, 2001)
"Two teams of laboratories...analyze thousands of newly discovered bacterial genes to try to discover the function of the 40-60% of them for which that is not known." (SciTech Book News, Vol. 25, No. 4, December 2001)