Rapid Cycle Real-Time PCR - Methods and Applications (eBook, PDF)
Quantification
Redaktion: Wittwer, Carl; Kaul, Karen; Hahn, Meinhard
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Rapid Cycle Real-Time PCR is a powerful technique for nucleicacid quantification and analysis that takes less than 30 minutesto complete. Fluorescence is automatically monitoredeach cycle and the amount of template quantified by advancedanalytical methods, such as the second derivative maximum method.Immediately following rapid cycle PCR, melting curve analysis isperformed to verify product purity with SYBR Green I and/orgenotype with fluorescently-labeled hybrdidization probes(HybProbes or SimpleProbes). Rapid cycle real-time PCR is oftencited as the most versatile, efficient method for nucle...
Rapid Cycle Real-Time PCR is a powerful technique for nucleic
acid quantification and analysis that takes less than 30 minutes
to complete. Fluorescence is automatically monitored
each cycle and the amount of template quantified by advanced
analytical methods, such as the second derivative maximum method.
Immediately following rapid cycle PCR, melting curve analysis is
performed to verify product purity with SYBR Green I and/or
genotype with fluorescently-labeled hybrdidization probes
(HybProbes or SimpleProbes). Rapid cycle real-time PCR is often
cited as the most versatile, efficient method for nucleic acid
quantification in research and climical studies. Molecular analysis
has never been easier!
acid quantification and analysis that takes less than 30 minutes
to complete. Fluorescence is automatically monitored
each cycle and the amount of template quantified by advanced
analytical methods, such as the second derivative maximum method.
Immediately following rapid cycle PCR, melting curve analysis is
performed to verify product purity with SYBR Green I and/or
genotype with fluorescently-labeled hybrdidization probes
(HybProbes or SimpleProbes). Rapid cycle real-time PCR is often
cited as the most versatile, efficient method for nucleic acid
quantification in research and climical studies. Molecular analysis
has never been easier!
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