eBook, PDF

Proteoglycan Protocols (eBook, PDF)

Beschreibung

Proteoglycans, highly complex macromolecules found in mammalian and lower organisms, serve a multitude of biological functions. In Proteoglycan Protocols, Renato Iozzo, MD, and an authoritative team of investigators present for the first time a comprehensive and up-to-date collection of readily reproducible preparative and analytical methods for the in-depth analysis of these important compounds. Featuring step-by-step protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, and to generate strategies for prokaryotic and eukaryotic expression. There are also detailed techniques for the suppression of specific proteoglycan genes, for the detection of mutant cells and their degradation products, and for studying specific interactions between proteoglycans and extracellular matrix proteins and with growth factors and their receptors. Each method is carefully described by leading experts in the field and includes practical considerations that are often lacking in formal papers. Authoritative and detailed, Proteoglycan Protocols offers molecular and cellular biologists, biochemists, pathologists, and geneticists a comprehensive reference and practical guide to the most common methods used today for the isolation and characterization of proteoglycans from cultured cells, tissues, and biological fluids.

Technische Details

• Geräte: PC
• ohne Kopierschutz
• Größe: 4.53MB

Barrierefreiheit

• Text-to-Speech

Produktdetails

• Verlag: Humana Press
• Seitenzahl: 558
• Erscheinungstermin: 2. Februar 2008
• Englisch
• ISBN-13: 9781592592098
• Artikelnr.: 64700358

Rezensionen

"The book is strategically subdivide into 3 sections (isolation and purification; expression, detection, and degradation; interactions) with a total of 51 chapters ( 19, 20, and 12 per subheading). Their contents dispel the clichés about work with sticky glycosaminoglycans being tedious, unrewarding, and mostly for the sake of old-fashioned...systematics....I expect even experts in certain areas to benefit from the whole book, whose rightful place will be on the bench, not on the shelf." - ChemBioChem "This book will appeal to both those new to the subject and experts in the field as the protocols outlined are both wide ranging, comprehensive, and easy to follow....strongly recommend it to those new to proteoglycan research, and would regard it as an essential addition to any proteoglycan research laboratory library." - Molecular Biotechnology "...this book will be a very useful tool for many specialists working in the fields of glycobiology, biotechnology, biochemistry, and experimental medicine. Detailed description of the methods employed for various aspects of proteoglycan analysis and comprehensive bibliography accompanying each chapter make this book especially valuable for a large audience of the mentioned specialists." - Biochemistry