Modern Applications of DNA Amplification Techniques (eBook, PDF)
eBook, PDF

Modern Applications of DNA Amplification Techniques (eBook, PDF)

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Redaktion: Lassner, Dirk; Rolfs, Arndt; Pustowoit, Barbara
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In the ten years since the first publication on PCR (Saiki et al. , 1985), this in vitro method of nucleic acid replication and modification has grown to rival in popularity traditional microbiological, genetical und technical procedures for cloning, sequencing, gene detecting and related procedures. To date the PCR literature has emphasized six main areas of application: genetic mapping, detection of mutations, genetic polymorphism, transcriptional splicing and regulation, molecular virology and quantitative procedures. The overwhelming focus of quantification of DNA or RNA by PCR has been on...

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