eBook, PDF

Fluorescence in situ Hybridization (FISH) (eBook, PDF)

Protocols and Applications

Redaktion: Bridger, Joanna M.; Morris, Keith

Beschreibung

The design simplicity and cost-effectiveness of the early Fluorescence in situ Hybridization (FISH) protocols, combined with the significant acceleration of discoveries in related technical areas like fluorescence microscopy, digital imaging, and nucleic acid technology have prompted the diversification of the original technique into an outstanding number of imaginative and useful applications, thus promoting its expansion into different areas of basic and applied research in the post-genomic era. In Fluorescence in situ Hybridization (FISH): Protocols and Applications, experts in the field portray the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of various applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology and evolutionary studies. The book is divided into four convenient sections covering the core techniques, technical advancements and novel adaptations, applications for human genetics and medicine, as well as protocols for model organisms. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab's current research.

Technische Details

• Geräte: PC
• ohne Kopierschutz
• Größe: 9.23MB

Barrierefreiheit

• Text-to-Speech

Produktdetails

• Verlag: Humana Press
• Seitenzahl: 451
• Erscheinungstermin: 2. September 2010
• Englisch
• ISBN-13: 9781607617891
• Artikelnr.: 64701808

Rezensionen

From the reviews:
"Fluorescence in situ hybridization (FISH) has been widely adopted to enable the study of uncultured target cells. This book shows many more applications in cytology. Relatively few chapters are devoted directly to microbiological applications; the strong theme of the book describes methods targeting chromosomes. ... Protocols on 3-D FISH may have useful material for microbiologists, e.g. studies on biofilms. ... I would suggest this is for microbiological libraries ... ." (Jonathan Porter, Microbiology Today, November, 2011)