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Expression of genes related to energy balance in adipose tissue of dairy cattle: effects of SCFA on mRNA abundance as quantified by qPCR and relevance of appropriate selection (eBook, PDF)

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The aim of this thesis was to study the effect of propionate (C3) and β-hydroxybutyrate (BHB) on the mRNA abundance of reference genes (RGs) and of the genes of interest (GOI) related to energy balance, by short term incubation (4 h) in bovine AT explants in vitro. Herein, we focused on three aspects: First, the differential effect of C3 and BHB on RGs and their use in gene expression normalization to improve the reliability of quantitative real-time PCR (qPCR) data. For this purpose, geNormTM and Normfinder© programs were used to identify the RGs with highest stability. The geometric m...

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