Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition (eBook, PDF)

Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition (eBook, PDF)

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Using a novel approach that combines high temporal resolution of the laser T-jump technique with unique sets of fluorescent probes, this study unveils previously unresolved DNA dynamics during search and recognition by an architectural DNA bending protein and two DNA damage recognition proteins.Many cellular processes involve special proteins that bind to specific DNA sites with high affinity. How these proteins recognize their sites while rapidly searching amidst ~3 billion nonspecific sites in genomic DNA remains an outstanding puzzle. Structural studies show that proteins severely deform DN...

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