Whole Genome Amplification
Methods Express
Herausgeber: Hughes, Simon; Lasken, Roger S.
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Genetic research is often hampered by a shortage of genomic DNA, particularly for high-throughput assays or when minute specimens or individual cells are being investigated. However, such problems can be overcome by using one of the latest whole genome amplification methods. These methods generate microgram quantities of DNA, from a starting sample of as little as a nanogram or even femtograms when studying a single bacterium. "Whole Genome Amplification: Methods Express" covers all of the well-established approaches, as well as the latest techniques, including: DOP-PCR - degenerated oligonucl...
Genetic research is often hampered by a shortage of genomic DNA, particularly for high-throughput assays or when minute specimens or individual cells are being investigated. However, such problems can be overcome by using one of the latest whole genome amplification methods. These methods generate microgram quantities of DNA, from a starting sample of as little as a nanogram or even femtograms when studying a single bacterium. "Whole Genome Amplification: Methods Express" covers all of the well-established approaches, as well as the latest techniques, including: DOP-PCR - degenerated oligonucleotide PCR I-PEP - improved primer extension pre-amplification; SCOMP - single cell comparative genomic hybridization GenomePlex whole genome amplification Linear amplification; and MDA - multiple displacement amplification. The book takes readers through all aspects of whole genome amplification, and provides the most rapid access possible to reliable protocols. For researchers using SNP analysis, comparative genomic hybridization, and microsatellite analysis, for example, "Whole Genome Amplification: Methods Express" is an essential guide to the approaches possible.
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