Viral Vectors for Gene Therapy
Gebundenes Buch

Viral Vectors for Gene Therapy

Methods and Protocols

Herausgegeben: Manfredsson, Fredric P.; Benskey, Matthew J.
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This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, construction, and application of transcription activation-like effectors; multi-modal production of adeno-associated virus; construction of oncolytic herpes simplex virus; AAV-mediated gene delivery to the mouse liver; and intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introdu...