Validation of Elisa for Testing of Antirabies Antibodies

Validation of Elisa for Testing of Antirabies Antibodies

Standardization and Validation of Indirect ELISA as a substitute for Virus Neutralization Test during Quantitative Testing of Antirabies Antibodies in Hyperimmunized Equines

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Indirect ELISA was evaluated for quantifying specific antirabies antibodies. A total of 4,946 samples of sixty equines were monitored by indirect ELISA at an interval of two weeks for a period of five years. When seven equines of the above sixty were compared for VNT and indirect ELISA during their primary phase of immunization, a good correlation was observed (r = 1.0). Based on this, the equines were segregated into ¿Low¿ responders, ¿Medium¿ responders and ¿High¿ responders. This segregation of equines based on their antibody titres proved to be very useful for initiating corrective r...