Ultracentrifugal analysis of biomolecular interaction processes
Christina Decker
Broschiertes Buch

Ultracentrifugal analysis of biomolecular interaction processes

Biophysical interaction analysis of proteins and nanoparticles using analytical ultracentrifugation

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As an absolute method in solution, analytical ultracentrifugation (AUC) enables the quantitative analysis of macromolecules based on their hydrodynamic separation in an applied centrifugal field. These fundamental properties of AUC allow extensive analysis of macromolecules in their native state under biologically relevant conditions and can provide information on their sedimentation coefficients, mass, shape, diffusion coefficients and density. Using gold nanoparticles and the two binding proteins amyloid-beta 42 and BSA, a generally valid procedure for an AUZ-based characterization of ligand...