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Commercial seaweed cultivation has been introduced to numerous parts of the world. Significant research is dedicated to its improvement of cultivation and processing. Kappaphycus alvarezii, a macroalgae, has spread throughout Southeast Asia and has been cultivated in many regions. The reliable cash income supports many coastal fishing communities. Separation of the solid-liquid fractions of fresh seaweed may provide additional value to seaweed farmers. Seaweed prices fluctuate due to variations in seaweed quality at the farm level. In addition, seaweed mostly grows better during the rainy season when there is a lack of sunlight to facilitate drying required for trade. If the seaweed can be immediately processed without drying and two products are obtained from liquid and solid separation, it will save space for drying and cost for adding new drying infrastructures. The liquid is used for agriculture fertilizer and the dry cake is used as an aquatic feed additive. Six factors werestudied to improve liquid extraction with mechanical expression using a fractional factorial design with two levels.