TET Proteins and DNA Demethylation
Broschiertes Buch

TET Proteins and DNA Demethylation

Methods and Protocols

Herausgegeben: Bogdanovic, Ozren; Vermeulen, Michiel
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This volume explores the latest methods used to study various aspects of TET proteins and their biology. Chapters in this book are divided into five parts. Part One describes technologies aimed at detecting and quantifying DNA methylation turnover using massively parallel sequencing, ELISA, and mass spectrometry approaches. Part Two looks at data analyses protocols for distinguishing acting versus passive DNA demethylation and estimation of 5mC and 5hmC levels. Part Three deals with a new topic that takes advantage of modified CRISPR/Cas9 genome editing systems to target DNA demethylation acti...