Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique including isolation techniques for DNA and RNA, gene cloning, PCR, ELISA, and cell culture. These protocols include a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves. This foundational reference is essential for students and researchers looking to enhance…mehr
Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique including isolation techniques for DNA and RNA, gene cloning, PCR, ELISA, and cell culture. These protocols include a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves. This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work.
Dr. Buddhi Prakash Jain is an Assistant Professor at the Department of Zoology, Mahatma Gandhi Central University, Motihari, Bihar, India. He completed his doctoral studies at the School of Life Sciences, Jawaharlal Nehru University (JNU) New Delhi, India. His research interests include gene expression regulation and endoplasmic reticulum stress in cancer. He is the recipient of several awards including a gold medal in Master Programme (M.Sc.), EMBO travel award, DST (Department of Science and Technology) Govt of India, Young Scientist travel award, and has published several papers in reputed international journals and a number of books and book chapters.
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1. Isolation of Genomic DNA: From animal cells, tissues, plant cell, and bacteria 2. Isolation of RNA 3. PCR (Polymerase Chain reaction) 4. Real-time (Quantitative) PCR 5. Electrophoresis 6. 3' RACE 7. 5'RACE 8. Isolation of Plasmid 9. Competent cell preparation 10. Gene Cloning 11. End labelling of DNA 12. SDS and NATIVE PAGE 13. Western Blotting 14. EMSA (Electrophoretic Mobility Shift Assay) 15. Silver staining 16. Cell Culture 17. MTT assay 18. Lactate Dehydrogenase assay 19. Luciferace assay 20. Co-IP 21. Protein purification 22. Sub cellular fractionation 23. DNA foot printing 24. Chromatin Immunoprecipitation 25. Fluorescent activated cell sorting (FACS) 26. Basics of Bioinformatics 27. GST Pulldown assay 28. Fluorescence In situ Hybridization 29. Yeast Two Hybrid Assay 30. ELISA (Enzyme Linked Immuno Sorbent Assay)