SSCP Conditions Optimization
Mohsin AminKhizra Bano
Broschiertes Buch

SSCP Conditions Optimization

Optimization of Single Strand Conformational Polymorphism (SSCP)Conditions for Exon 4-12 of LDLR Gene

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Single strand conformational polymorphism (SSCP) is a technique which is used to detect point mutation in genes. The purpose was to optimize the condition of SSCP for efficient and sensitive mutation detection in Exon 4, 5, 6, 7, 8, 9, 10, 11 and 12. All these Exon were amplified and run on agarose gel to check strong and specific amplification. These samples were then subjected for vertical electrophoresis for SSCP using different conditions of gel concentration, time, temperature and voltage to get maximum separation between single strands. Denaturation was carried out by mixing the samples ...