Xuan Ou

Broschiertes Buch

SIRT1 DEFICIENCY COMPROMISES EMBRYONIC AND ADULT HEMATOPOIESIS IN MICE

SIRT1 DEFICIENCY COMPROMISES MOUSE EMBRYONIC STEM CELL DIFFERENTIATION, AND EMBRYONIC AND ADULT HEMATOPOIESIS IN MICE

Beschreibung

SIRT1 is a founding member of a sirtuin family of seven proteins and histone deacetylases. It is involved in cellular resistance to stress, metabolism, differentiation, aging, and tumor suppression. SIRT1-/- mice demonstrate embryonic and postnatal development defects. We examined hematopoietic and endothelial cell differentiation of SIRT1-/- mouse embryonic stem cells (ESCs) in vitro, and hematopoietic progenitors in SIRT1+/+, +/-, and -/- mice. SIRT1-/- ESCs formed fewer mature blast cell colonies. Replated SIRT1-/- BL-CFCs demonstrated defective hematopoietic potential. Endothelial cell production was unaltered, but there were defects in formation of a primitive vascular network from SIRT1-/- derived embryoid bodies. Development of primitive and definitive progenitors derived from SIRT1-/- ESCs were also delayed and/or defective. Differentiation delay/defects were associated with delayed capacity to switch off Oct4, Nanog and Fgf5 expression, decreased -H1 globin, -major globin, and Scl gene expression, and reduced activation of Erk1/2. Ectopic expression of SIRT1 rescued SIRT1-/- ESC differentiation deficiencies.

Autor

Xuan Ou Doktor der Philosophie, Abteilung für Mikrobiologie und Immunologie, Indiana University, Indianapolis, IN. Postdoc-Wissenschaftler bei Eli Lilly and Company, Indianapolis IN.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 152
• Erscheinungstermin: 29. Juni 2011
• Englisch
• Abmessung: 220mm x 150mm x 10mm
• Gewicht: 218g
• ISBN-13: 9783844389654
• ISBN-10: 3844389652
• Artikelnr.: 33688632