Gebundenes Buch

Single-Cell Omics in Microbiome Research

Exploring Microbial Diversity Across Ecosystems

Herausgeber: Rinke, Christian; D¿unková, Mária

Beschreibung

This volume introduces single-cell microbial genomics as an innovative approach to the study of microbial diversity and symbiosis that aims to unravel the complex composition and interactions of environmental microbiomes. The reader is guided through the typical workflow of the method and is given a detailed insight into how bacterial cells of interest are first sorted using fluorescence-activated cell sorting (FACS), how the collected microbial DNA is amplified and sequenced using shotgun sequencing to generate single amplified genomes (SAGs), which can then be analysed for their phylogeny, taxonomy and metabolic characteristics or studied for microbial interactions. In addition, the book provides an overview of advances in the field of microbial single-cell genomics, which expand its use over single bacterial and archaeal cells to co-sorted microbial cells and even single viruses. The methods used to achieve these results, including a variety of fluorescent probes and alternative labelling approaches, are presented. The book is aimed at researchers and students of environmental microbiology who wish to expand their knowledge of this ground-breaking technology.

Autor

Christian Rinke received his PhD in Zoology from the Marine Biology Department at the University of Vienna, Austria and has since shifted his focus to the microbial world. His research interests include the phylogeny, taxonomy and ecology of free living and symbiotic Bacteria and Archaea. Thereby, he focuses on the majority of microbes which elude current culturing efforts. This so-called "Microbial Dark Matter" can only be explored with culture-independent approaches, and hence Chris has pioneered methods in high throughput single-cell genomics, the separation and sequencing of single bacterial and archaeal cells, and metagenomics, the direct sequencing of DNA from environmental samples. Currently, his lab uses a range of "omics" techniques to learn more about the phylogenetic and metabolic diversity encoded in Microbial Dark Matter. He is also a member of the Genome Taxonomy Database (GTBD) curation team. GTDB is an initiative to establish a standardised microbial taxonomy based on genome phylogeny. Mária Dunková received her PhD in Biotechnology from the University of Valencia, Spain, where she focused on integrating flow cytometry with metagenomics to study the human gut microbiome. She gained further experience as a predoctoral researcher at the Czech Academy of Sciences and Harvard Medical School, and later as a postdoctoral researcher at the Australian Centre for Ecogenomics, University of Queensland (2016–2019), and the DOE Joint Genome Institute at Lawrence Berkeley National Laboratory (2019–2021). In 2021, she joined the Institute for Integrative Systems Biology (I2SysBio) at the University of Valencia, where she established the Microbial Single-Cell Genomics research group. Her work combines metagenomics and single-cell genomics to uncover novel microbial lineages and elucidate their functions without the need for cultivation. She is particularly interested in symbiotic interactions between soft-bodied marine animals and their microbes producing protective molecules, microbial life in extreme industrial environments, and horizontal gene transfer driven by phages and other mobile genetic elements.

Produktdetails

• Verlag: Springer International Publishing AG / Springer-Verlag GmbH
• Artikelnr. des Verlages: 89283612
• Erscheinungstermin: 19. Dezember 2025
• Englisch
• Abmessung: 235mm x 155mm
• ISBN-13: 9783032075260
• ISBN-10: 3032075262
• Artikelnr.: 75303831