Simultaneous estimation of antihypertensive drugs by HPLC
Determination of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide using RP-HPLC
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Every year many new drugs and newer drug combinations enter the pharmaceutical area. Analytical methods for these new and first timer drugs are mostly confined only to the manufacturing company. However, availability of multiple analytical methods for the same drug/drug combinations in their formulations is always advantageous. Moreover, development of such methods helps in training the analysts for skillfully handling the sophisticated analytical instruments and the way for research approach. The present work is also planned on similar lines. A simple, precise, rapid and accurate Reversed-Pha...
Every year many new drugs and newer drug combinations enter the pharmaceutical area. Analytical methods for these new and first timer drugs are mostly confined only to the manufacturing company. However, availability of multiple analytical methods for the same drug/drug combinations in their formulations is always advantageous. Moreover, development of such methods helps in training the analysts for skillfully handling the sophisticated analytical instruments and the way for research approach. The present work is also planned on similar lines. A simple, precise, rapid and accurate Reversed-Phase High Performance Liquid Chromatography method is developed for the simultaneous estimation of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide in dosage forms. Chromatography is carried out isocratically at 25°C ± 0.5°C on Liquid chromatographic system from Young Lin 9100 comprising of hyperchrome C-18 column (4.6 x 250mm, 5 particle size), manual injector, YL 9110 quaternary pump and Photodiode array YL 9160 detector connected to software YL clarity. Parameters such as linearity, precision, accuracy, recovery and specificity are studied as reported in the ICH guidelines.
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