Restriction Fragment Length Polymorphism
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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. In molecular biology, the term restriction fragment length polymorphism, or RFLP, (commonly pronounced rif-lip ) refers to a difference between two or more samples of homologous DNA molecules arising from differing locations of restriction sites, and to a related laboratory technique by which these segments can be distinguished. In RFLP analysis the DNA sample is broken into pieces (digested) by restriction enzymes and the resulting restriction fragments are separated...
Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. In molecular biology, the term restriction fragment length polymorphism, or RFLP, (commonly pronounced rif-lip ) refers to a difference between two or more samples of homologous DNA molecules arising from differing locations of restriction sites, and to a related laboratory technique by which these segments can be distinguished. In RFLP analysis the DNA sample is broken into pieces (digested) by restriction enzymes and the resulting restriction fragments are separated according to their lengths by gel electrophoresis. Although now largely obsolete, RFLP analysis was the first DNA profiling technique inexpensive enough to see widespread application. In addition to genetic fingerprinting, RFLP was an important tool in genome mapping, localization of genes for genetic disorders, determination of risk for disease, and paternity testing.
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