Recombinant Protein Production in Yeast
Broschiertes Buch

Recombinant Protein Production in Yeast

Methods and Protocols

Herausgegeben: Bill, Roslyn M.
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In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, op...