Renato V. Iozzo (ed.)

Gebundenes Buch

Proteoglycan Protocols

Beschreibung

Proteoglycans are some of the most elaborate macromolecules of mammalian and lower organisms. The covalent attachment of at least five types of glycosami- glycan side chains to more than forty individual protein cores makes these molecules quite complex and endows them with a multitude of biological functions. Proteoglycan Protocols offers a comprehensive and up-to-date collection of prepa- tive and analytical methods for the in-depth analysis of proteoglycans. Featuring st- by-step detailed protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, to generate strategies for prokaryotic and eukaryotic expression, to utilize methods for the suppression of specific proteoglycan gene expression and for the detection of mutant cells and degradation products, and to study specific interactions between proteoglycans and extracellular matrix proteins as well as growth factors and their receptors. The readers will find concise, yet comprehensive techniques carefully drafted by leading experts in the field. Each chapter commences with a general Introduction, followed by a detailed Materials section, and an easy-to-follow Methods section. An asset of each chapter is the extensive notation that includes troubleshooting tips and practical considerations that are often lacking in formal methodology papers. The reader will find this section most valuable because it is clearly provided by experienced scientists who have first-hand knowledge of the techniques they outline. In addition, most of the chapters are well illustrated with examples of typical data generated with each method.

Produktdetails

• Methods in Molecular Biology 171
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 978-0-89603-759-5
• 2001
• Seitenzahl: 580
• Erscheinungstermin: 1. Juni 2001
• Englisch
• Abmessung: 235mm x 157mm x 36mm
• Gewicht: 1016g
• ISBN-13: 9780896037595
• ISBN-10: 0896037592
• Artikelnr.: 22447015

Rezensionen

"The book is strategically subdivide into 3 sections (isolation and purification; expression, detection, and degradation; interactions) with a total of 51 chapters ( 19, 20, and 12 per subheading). Their contents dispel the clichés about work with sticky glycosaminoglycans being tedious, unrewarding, and mostly for the sake of old-fashioned...systematics....I expect even experts in certain areas to benefit from the whole book, whose rightful place will be on the bench, not on the shelf." - ChemBioChem "This book will appeal to both those new to the subject and experts in the field as the protocols outlined are both wide ranging, comprehensive, and easy to follow....strongly recommend it to those new to proteoglycan research, and would regard it as an essential addition to any proteoglycan research laboratory library." - Molecular Biotechnology "...this book will be a very useful tool for many specialists working in the fields of glycobiology, biotechnology, biochemistry, and experimental medicine. Detailed description of the methods employed for various aspects of proteoglycan analysis and comprehensive bibliography accompanying each chapter make this book especially valuable for a large audience of the mentioned specialists." - Biochemistry