Protein microarray synthesis and label-free interaction analysis
Jürgen Burger
Broschiertes Buch

Protein microarray synthesis and label-free interaction analysis

Design and evaluation of automated system

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Protein microarrays are essential tools for high-throughput proteome interaction and binding kinetics analysis. Recent technical developments have resulted in approaches for the generation of protein arrays by in situ cell-free expression of DNA array templates. We combined and enhanced these ideas by creating innovative microfluidic flow cells in standard microscope slide format and such improved system efficiency, operability, robustness and controllability. An automated setup suitable for both the in situ expression as well as the interaction analysis of protein microarrays by flow-injectio...