Preparation of an Affinity Chromatographic Matrix
Md. Tanvir Hossain
Broschiertes Buch

Preparation of an Affinity Chromatographic Matrix

For the Selective Purification of the Maltose Specific Lectin

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Affinity media with immobilized carbohydrates have been widely used for the separation of lectins in the final purification step in the multistep protocols. These affinity matrixes themselves are fairly expensive and contribute to the high purification costs. The present work shows a simple and inexpensive method for the preparation of an affinity chromatographic matrix for maltose-specific lectins. Agarose beads were prepared by emulsification technique where 4% w/v agarose was dissolved in boiling water containing 0.9% NaCl and used as water phase. A mixture of liquid paraffin containing 3 w...