• Produktbild: PCR Cloning Protocols
  • Produktbild: PCR Cloning Protocols
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PCR Cloning Protocols

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Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

10.11.2010

Herausgeber

Bing-Yuan Chen + weitere

Verlag

Humana Press

Seitenzahl

439

Maße (L/B/H)

23,5/15,5/2,5 cm

Gewicht

686 g

Auflage

2nd Edition.

Sprache

Englisch

ISBN

978-1-61737-281-0

Beschreibung

Rezension

"This hardcover book is packed full of detailed protocols and methodological articles on various aspects of PCR. The fact that the book was taken out of my hands by a colleague, to read up on touch-down PCR, as soon as I received it for review, attest to the book's usefulness. I will have to chain this book to my bookshelf, to make sure it does not disappear quickly-or I may consider buying a couple for the laboratory." -Plant Science

Review of the first edition.

This volume provides protocols useful to both the inexperienced and the experienced investigator. Most importantly it highlights the emerging role of PCR as a versatile tool for the molecular biologist eliminating many laborious and expensive techniques associated with traditional gene isolation and analysis.-Biochemical Education

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

10.11.2010

Herausgeber

Verlag

Humana Press

Seitenzahl

439

Maße (L/B/H)

23,5/15,5/2,5 cm

Gewicht

686 g

Auflage

2nd Edition.

Sprache

Englisch

ISBN

978-1-61737-281-0

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: [email protected]

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  • Produktbild: PCR Cloning Protocols
  • Produktbild: PCR Cloning Protocols
  • Part I. Performing and Optimizing PCR

    Polymerase Chain Reaction: Basic Principles and Routine Practice
    Lori A. Kolmodin and David E. Birch

    Computer Programs for PCR Primer Design and Analysis
    Bing-Yuan Chen, Harry W. Janes, and Steve Chen

    Single-Step PCR Optimization Using Touchdown and Stepdown PCR Programming
    Kenneth H. Roux

    XL PCR Amplification of Long Targets from Genomic DNA
    Lori A. Kolmodin

    Coupled One-Step Reverse Transcription and Polymerase Chain Reaction Procedure for Cloning Large cDNA Fragments
    Jyrki T. Aatsinki

    Long Distance Reverse-Transcription PCR
    Volker Thiel, Jens Herold, and Stuart G. Siddell

    Increasing PCR Sensitivity for Amplification from Paraffin-Embedded Tissues
    Abebe Akalu and Juergen K. V. Reichardt

    GC-Rich Template Amplification by Inverse PCR: DNA Polymerase and Solvent Effects
    Alain Moreau, Da Shen Wang, Steve Forget, Colette Duez, and Jean Dusart

    PCR Procedure for the Isolation of Trinucleotide Repeats
    Teruaki Tozaki

    Methylation-Specific PCR
    Haruhiko Ohashi

    Direct Cloning of Full-Length Cell Differentially Expressed Genes by Multiple Rounds of Subtractive Hybridization Based on Long-Distance PCR and Magnetic Beads
    Xin Huang, Zhenglong Yuan, and Xuetao Cao

    Part II. Cloning PCR Products

    Cloning PCR Products: An Overview
    Baotai Guo and Yuping Bi

    Using T4 DNA Polymerase to Generate Clonable PCR Products
    Kai Wang

    Enzyme-Free Cloning of PCR Products and Fusion Protein Expression
    Brett A. Neilan and Daniel Tillett

    Directional Restriction Site-Free Insertion of PCR Products into Vectors
    Guo Jun Chen

    Autosticky PCR: Directional Cloning of PCR Products with Preformed 5' Overhangs
    József Gál and Miklós Kálmán

    A Rapid and Simple Procedure for Direct Cloning of PCRProducts into Baculoviruses
    Tamara S. Gritsun, Michael V. Mikhailov, and Ernest A. Gould

    Part III. Mutagenesis and Recombination

    PCR Approaches to DNA Mutagenesis and Recombination: An Overview
    Binzhang Shen

    In-Frame Cloning of Synthetic Genes Using PCR Inserts
    James C. Pierce

    Megaprimer PCR
    Sailen Barik

    PCR-Mediated Recombination: A General Method Applied to Construct Chimeric Infectious Molecular Clones
    Guowei Fang, Barbara Weiser, Aloise Visosky, Timothy Moran, and Harold Burger

    PCR Method for Generating Multiple Mutations at Adjacent Sites
    Jiri Adamec

    A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing Repeat Expansions into CAG-Repeat Containing Genes
    Franco Laccone

    PCR Screening in Signature-Tagged Mutagenesis of Essential Genes
    Dario E. Lehoux and Roger C. Levesque

    Staggered Extension Process (StEP) In Vitro Recombination
    Anna Marie Aguinaldo and Frances Arnold

    Random Mutagenesis by Whole-Plasmid PCR Amplification
    Donghak Kim and F. Peter Guengerich

    Part IV. Cloning Unknown Neighboring DNA

    PCR-Based Strategies to Clone Unknown DNA Regions from Known Foreign Integrants: An Overview
    Eric Ka-Wai Hui, Po-Ching Wang, and Szecheng J. Lo

    Long Distance Vectorette PCR (LDV PCR)
    James A. L. Fenton, Guy Pratt, and Gareth J. Morgan

    Nonspecific, Nested Suppression PCR Method for Isolation of Unknown Flanking DNA ('Cold-Start Method')
    Michael Lardelli

    Inverse PCR: cDNA Cloning
    Sheng-He Huang

    Inverse PCR: Genomic DNA Cloning
    Ambrose Y. Jong, Anna T'ang, De-Pei Liu, and Sheng-He Huang

    Gene Cloning and Expression Profiling by Rapid Amplification of Gene Inserts with Universal Vector Primers
    Sheng-He Huang, Hua-Yang Wu, and Ambrose Y. Jong

    The Isolation of DNA Sequences Flanking Tn5 Trans