Osmotic tolerance of goat semen and freezing in medium without glycerol

Osmotic tolerance of goat semen and freezing in medium without glycerol

Survival in sucrose solution, freezing and cryoprotectant

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In this book, Dr. Sandra Cristina Becker-Silva presents a revolutionary investigation into the cryopreservation of goat semen, the fruit of her academic research. The author explores the limits of sperm tolerance to hyperosmotic sucrose solutions and their survival after cryopreservation, challenging conventional methods.Through meticulous experiments, Becker-Silva develops innovative freezing protocols. The research reveals that partial dehydration with concentrated sucrose or trehalose solutions allows goat semen to be frozen without permeating cryoprotectants, a significant advance in the f...