Ordered DNA Fragmentation and Amplification for NGS Applications
NaHyun Cho
Broschiertes Buch

Ordered DNA Fragmentation and Amplification for NGS Applications

Fragmentation of Surface Adsorbed and Aligned DNA Molecules using Soft Lithography for NGS applications

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In this study, the enzymatic in situ cutting of linearized DNA molecules at approximately 11kbp intervals is demonstrated using a soft lithography technique. The ultimate goal is to provide a general ordered cutting method to greatly simplify the assembly process of NGS systems by reducing computational reassembly complexity. DNA was stretched onto PMMA (Poly methyl methacrylate) coated silicon by withdrawing the substrate from a DNA solution (a process termed "combing"). The stretched lambda DNA could be linearly cut with a soft lithography stamp used to selectively apply DNase I. After cutti...