Nanoscale Imaging and Characterisation of Amyloid-¿

Nanoscale Imaging and Characterisation of Amyloid-¿

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This thesis presents a method for reliably and robustly producing samples of amyloid-beta (Abeta) by capturing them at various stages of aggregation, as well as the results of subsequent imaging with various atomic force microscopy (AFM) methods, all of which add value to the data gathered by collecting information on the peptide's nanomechanical, elastic, thermal or spectroscopical properties. Amyloid-beta (Abeta) undergoes a hierarchy of aggregation following a structural transition, making it an ideal subject of study using scanning probe microscopy (SPM), dynamic light scattering (DLS) and...