Mycobacteriology in low-income countries
Development of simple and inexpensive tools for the control of tuberculosis and Buruli ulcer
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Tuberculosis and Buruli ulcer are the most important mycobacterial diseases in several low-income countries. To control the diseases, these countries have to face many challenges such as lack of facilities and/or funds. Therefore, there is a great need of simple, rapid and inexpensive tools to control these diseases. For tuberculosis, the tools developed in this book include bulk staining method for microscopy, a resazurin microplate assay for differentiation of Mycobacterium tuberculosis from nontuberculous mycobacteria and nitrate reductase assays for detection of drug resistance. For Buruli...
Tuberculosis and Buruli ulcer are the most important mycobacterial diseases in several low-income countries. To control the diseases, these countries have to face many challenges such as lack of facilities and/or funds. Therefore, there is a great need of simple, rapid and inexpensive tools to control these diseases. For tuberculosis, the tools developed in this book include bulk staining method for microscopy, a resazurin microplate assay for differentiation of Mycobacterium tuberculosis from nontuberculous mycobacteria and nitrate reductase assays for detection of drug resistance. For Buruli ulcer, an experience of setting up a national reference laboratory as well as a simple smear preparation method for microscopy are presented. Finally, a simple algorithm for the organisation of Buruli ulcer diagnosis in endemic settings is proposed.
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