Multiphoton Imaging of the Spatial and Temporal Patterns of Transient Starch and Grana in Arabidopsis Seedling
Ping-Chih Yang
Broschiertes Buch

Multiphoton Imaging of the Spatial and Temporal Patterns of Transient Starch and Grana in Arabidopsis Seedling

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We took advantage of starch, which emits intense SHG (second harmonic generation) radiation uponthe application of optical excitation sources, to noninvasively monitor germination process of starch in Arabidopsis postembryos for the course of 5 days and some later phases of the seedling development. We presented the temporal and spatial distribution patterns of a whole Arabidopsis seedling in a more natural, noninvasive manner. We can further derive the developmental state of every cell in the seedling. At last we also examined some later phases of the evolution of Arabidopsis to demonstrate t...