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Microwave Assisted Proteomics
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This book is an introduction to microwave-assisted bio-analytical methods with an emphasis on sample preparation for proteomic analyses. Its objective is to provide a history and background for the methods described and the book summarizes the development and application of these protocols from both the literature in this field as well as from the experiences of a variety of laboratories conducting microwave-assisted protocols. In addition to describing the evolution of the microwave-assisted methodologies, it includes mechanisms of action as well as an overview of the types of instrumentation...
This book is an introduction to microwave-assisted bio-analytical methods with an emphasis on sample preparation for proteomic analyses. Its objective is to provide a history and background for the methods described and the book summarizes the development and application of these protocols from both the literature in this field as well as from the experiences of a variety of laboratories conducting microwave-assisted protocols. In addition to describing the evolution of the microwave-assisted methodologies, it includes mechanisms of action as well as an overview of the types of instrumentation available to help the reader develop the best experimental design for their own budget and experimental aims. The key features of this book: * An overview of the history of microwave-assisted proteomics * An in-depth discussion on several microwave-assisted methods including - enzymatic and chemical proteolytic reactions, microwave-assisted acid hydrolysis for N-terminal sequencing and for amino acid analysis * A discussion of the mechanisms involved * An overview of instrumentation types and limitations * A concise and practical protocol section for the researcher to carry out the experiments in their own laboratory Microwave-assisted proteomics is a rapidly evolving field and this book captures the main areas that fall under this emerging arena.
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