Meristem Culture of Apple Cultivar ¿Oregon Spur-II¿

Meristem Culture of Apple Cultivar ¿Oregon Spur-II¿

Meristem Culture of Apple

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An attempt was made to eliminate ACLSV, ApMV, ASGV and ASPV from apple cultivar 'Oregon Spur-II'. During establishment of explants, highest survival percentage (62.35%) and proliferation (30.68%) was recorded during summer season, due to less browning and contamination. However, size of meristems and position of buds from where meristems were excised also influenced their survival. The meristems of size 0.6 - 0.7 mm were found to be the most appropriate for maximum establishment i.e., 36.36 %. Meristems excised from buds positioned on distil portions of actively growing shoots showed better re...