M. Chance

Gebundenes Buch

Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics

Beschreibung

A how-to guide that helps you take advantage of the latest mass spectrometry methods

This book brings together and examines the latest mass spectrometry methods used to investigate proteins, including structural mechanisms, protein dynamics, and interactions among proteins. Written by a team of experts with many years of hands-on experience developing and working with mass spectrometry methods, the book offers a wealth of practical advice and tips to help readers fully exploit the capabilities of a mass spectrometer.

The book begins with an overview of mass spectrometry technologies, including an expert forecast of future directions. Next, the authors offer step-by-step guidance for such topics as:
_ Complementary methods for structure determination: hydroxyl radical-mediated footprinting and deuterium exchange mass spectrometry as applied to serpin structure
_ Covalent labeling methods for examining protein structure and protein interactions
_ Computational approaches to examine protein-protein interactions: combining experimental and computational data in the era of structural genomics
_ Two approaches to mass spectrometric protein footprinting: PLIMSTEX and FPOP
_ Hydrogen/deuterium exchange studies of viruses

Carefully edited, the book presents a uniform standard of high quality and thoroughness throughout all the chapters. References to the literature enable readers to explore each individual topic in greater depth.

Any researcher seeking to advance his or her own protein research will benefit from this book's insights and how-to guidance. The spectrometry methods can be applied to support investigations in such fields as biomedicine, biotechnology, and pharmaceutical research.

Autor

Mark Chance, PhD, is the Director of the Case Center for Proteomics and Professor of Physiology and Biophysics at Case Western Reserve University. He also directs the Case Center for Synchrotron Biosciences, located at the National Synchrotron Light Source at Brookhaven National Laboratory, New York. Dr. Chance received his bachelor's degree in biology from Wesleyan University and his PhD in biophysics from the University of Pennsylvania. He furthered his training as a postdoctoral research associate at AT&T Bell Laboratories, then went on to become assistant professor in the department of chemistry at Georgetown University. He moved to Albert Einstein College of Medicine in the Bronx, New York, in 1992, and was soon promoted to associate professor of the departments of physiology & biophysics and biochemistry, and full professor in 1998. While at AECOM, he was the Joseph and Anne Wunsch fellow in biophysical engineering and recipient of the Irma Hirschl Career Scientist Award. In 2005, he moved to Case Western Reserve University as the founding director of the Center for Proteomics. His research primarily concerns development of structural and cellular proteomics technologies to understand protein structure-function.

Produktdetails

• Verlag: Wiley & Sons
• 1. Auflage
• Seitenzahl: 304
• Erscheinungstermin: 22. September 2008
• Englisch
• Abmessung: 236mm x 160mm x 20mm
• Gewicht: 564g
• ISBN-13: 9780470258866
• ISBN-10: 0470258861
• Artikelnr.: 23878583