Lysine production by Corynebacteruim glutamicum Homoserine (-)
Effect of ammonium acetate on the productivity of an auxotrophic mutant
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The aim of our work is to study the effect of ammonium acetate as a carbon and nitrogen source on the productivity of homoserine (-) auxotrophic Corynebacterium glutamicum mutants. To this end, we inoculated four media containing a homoserine (-) auxotrophic C.glutamicum mutant as a carbon source (glucose; glucose and ammonium acetate; ammonium acetate; ammonium acetate and sodium acetate). During this fermentation, we monitored biomass production (by turbidimetry) and lysine production by the strain, while measuring glucose and ammonium acetate consumption. We found that in an ammonium acetat...
The aim of our work is to study the effect of ammonium acetate as a carbon and nitrogen source on the productivity of homoserine (-) auxotrophic Corynebacterium glutamicum mutants. To this end, we inoculated four media containing a homoserine (-) auxotrophic C.glutamicum mutant as a carbon source (glucose; glucose and ammonium acetate; ammonium acetate; ammonium acetate and sodium acetate). During this fermentation, we monitored biomass production (by turbidimetry) and lysine production by the strain, while measuring glucose and ammonium acetate consumption. We found that in an ammonium acetate-based medium, fermentation was characterized by remarkable lysine production (11.45 g/l) and low biomass production.Key words: Corynebacterium glutamicum, auxotrophs, homoserine(-), mutation, ammonium acetate, glucose, lysine, feedback inhibition.
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