Isolation and characterization of non-rhizobial endophytic bacteria

Isolation and characterization of non-rhizobial endophytic bacteria

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Present investigation was carried out to isolate efficient non rhizobial root nodule endophytic PGPB from leguminous crops viz. Glycine max (soybean), Vigna radiata (mung bean), Vigna unguiculata (cow pea), Arachis hypogaea (groundnut) and Vigna mungo (black gram) from AAU, Anand, Gujarat. Out of thirty four, fourteen bacterial isolates were able to grow and flourish on nitrogen free medium. These fourteen isolates were further subjected to amplification of nifH gene and seven were found to be amplifying nifH gene of size ~380 bp which by 16S rRNA sequencing characterized as Enterobacter cloac...