Isolation and Characterization of Moderate Thermophilic Bacteria
Aruna Sharmili S.
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Isolation and Characterization of Moderate Thermophilic Bacteria

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The present work is the first report of moderate thermophilic bacteria from the coastal waters of Coramandal coast, Chennai, Tamilnadu. Of the 334 bacterial isolates, only 44 isolates were found to grow up to 55°C. The isolates showed highest incidence of antibiotic resistance to most of the antibiotics tested. Polyphasic approach viz., biochemical tests, Fatty Acid Methyl Ester Analysis (FAME), SDS-PAGE analysis, 16S ribosomal RNA gene analysis and Scanning Electron Microscopy (SEM) was adopted to identify the moderately thermophilic bacterial isolates. Biochemical tests indicated the isolat...