Isolation and Characterization of Biosurfactant-Producing Bacteria
Israa S. Al-Rowaihi
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Isolation and Characterization of Biosurfactant-Producing Bacteria

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Petroleum-polluted environmental samples were collected to isolate and characterize biosurfactant-producing bacteria. Culturing of the collected samples in LB-broth and enrichment in chemically defined medium (CDM) with various carbon sources yielded 51 strains, some of which produced biosurfactants/bioemulsifiers when challenged with crude oil as a sole carbon source. The I-15 isolate, a Gram-positive, motile bacillus, emulsified crude oil in CDM without reduction in surface tension. Infrared spectroscopy and thin layer chromatography suggested a lipopeptide structure for the crude biosurfact...