Identification of ABA-binding proteins by photoaffinity labeling
Marek Galka
Broschiertes Buch

Identification of ABA-binding proteins by photoaffinity labeling

Marek Galka London, ON 2011

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The primary objective of this project was isolation and identification of new ABA-binding proteins in plants. This was to be achieved by employing a biologically active ABA derivative, which served as both an affinity and a photoaffinity probe. It has been proven that this probe was recognized by known ABA-binding proteins. Subsequently the probe was used to isolate putative ABA binding proteins, and this led to the discovery of Rubisco as a new ABA binding protein, among several other potential candidates. The binding of ABA to Rubisco was confirmed by various techniques and it was found that...